Microbiology

What is Dark Field Microscopy? Definition of Dark Field Microscope A darkfield microscope is a type of optical microscope that uses oblique illumination to light the specimen, causing it to appear bright against a dark background. This technique enhances the visibility of transparent and living specimens by excluding the unscattered light from the image. Principle … Read more

The concept of using fluorescence to study biological materials dates back to the late 19th century, but the first practical fluorescence microscope was not developed until the 1930s. In 1938, Robert Goldstein and Richard Manly, researchers at the University of California, Berkeley, published a paper describing the use of fluorescence to study cells and tissues … Read more

What is Oil Immersion? What is Microscopic Resolution? Microscopic Resolution Definition The microscopic resolution also is known as resolving power. Resolution is defined as the ability of a lens to separate or distinguish between small objects that are close together or the ability to distinguish two adjacent points as distinct and separate. Equation of Microscopic … Read more

What is transmission electron microscope? Parts of A Transmission Electron Microscope 1. Electron Gun Types of Electron Gun Used in Transmission Electron Microscope There are several types of electron guns that are used in transmission electron microscopes (TEMs), including: Overall, the type of electron gun used in a TEM depends on the specific requirements and … Read more

A scanning electron microscope (SEM) is a type of electron microscope that generates images by scanning the surface of a specimen with a concentrated beam of electrons. Electrons interact with atoms in the sample, generating signals that carry information about the sample’s surface topography and composition. The electron beam is scanned in a raster scan … Read more

What is phase contrast microscopy? A phase contrast microscopy converts slight differences in refractive index and cell density into easily detected variation in light intensity to observe living cells. This microscope is used for visualization of cell culture and live cells. Living cells can be observed without any staining. Unstained specimens have absorbed no light, … Read more

Confocal microscopy has various advantages over conventional optical microscopy, including a short depth of field, the elimination of out-of-focus glare, and the ability to collect optical slices serially from thick specimens. A prominent use of confocal microscopy in the biomedical sciences is the imaging of either fixed or live cells and tissues that have typically … Read more

Stereo microscopes, in contrast to compound light microscopes, often employ reflected illumination, or light reflected off an object’s surface, rather than transmitted (diascopic) illumination. When a specimen is too thick or opaque for compound microscopy, reflected light from the object enables for investigation. Unlike a compound microscope, transmitted illumination in most stereo microscopes is not … Read more

A microscope is a scientific instrument that is used to magnify the image of an object or sample in order to study its structure or features in detail. Microscopes are commonly used in scientific and medical research, as well as in education and other applications, to study small structures and organisms that are not visible … Read more