Different Aseptic Techniques in Microbiology Laboratory

Different Aseptic Techniques in Microbiology Laboratory

Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.

Spread‌ ‌Plate‌ ‌Method‌ – Procedure, Principle, Purpose, Result.

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more

Pour Plate Method – Principle, Procedure, Objective, Result, Advantages

Pour Plate Method Principle, Procedure, Objective, Result

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more

Subculturing Technique In Agar Slant/Agar Plate

Subculturing Technique

Overview of Subculturing Technique In microbiology, subculture is defined as a new cell or microbiological culture, which is prepared by transferring a few or all cells to a fresh growth medium from an old culture or a previous culture. In a single word, the transfer of cells from a previous culture to a fresh or … Read more

Subculturing technique for Broth Culture

Subculturing technique for Broth Culture

Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more

Streak Plate Method – Patterns, Procedure, Principle

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species.  During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more

Fructose Metabolism Pathway – Fructolysis

Fructose Metabolism Pathway - Fructolysis

Location of Fructolysis Why does fructose metabolism or fructolysis occur in liver cells? Properties and Sources of Fructose Absorption of Fructose From Fructose to Fat/Fructolysis/fructose metabolism pathway Steps of fructose metabolism pathway 1. Step 1 2. Step 2 3. Step 3A Metabolic Pathway Notes: At this point, the liver cell can use the same steps … Read more

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2)

What is Photosystem 1 (PS1)? What is Photosystem 2 (PS2)? Difference between Photosystem 1 (PS1) and Photosystem 2 (PS2) Feature Photosystem I (PSI) Photosystem II (PSII) Location in Thylakoid Membrane Outer surface Inner surface Photocenter P700 P680 Absorbing Wavelength >680 nm <680 nm Photophosphorylation Both cyclic and non-cyclic Only non-cyclic Photolysis of Water No Yes … Read more

Top 15 Books for Molecular Biology

Top 15 Books for Molecular Biology

In this article, I will share a list of Top and Best Molecular Biology Books. In this paper on microbiology, you will study about Structures of DNA and RNA / Genetic Material,  Replication of DNA (Prokaryotes and Eukaryotes), Transcription in Prokaryotes and Eukaryotes, Post-Transcriptional Processing, Translation (Prokaryotes and Eukaryotes), Regulation of gene Expression in Prokaryotes … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more