Iodine Test – Principle, Procedure, Result, Uses

Iodine Test - Iodine test for starch Principle, Procedure, Result, Uses

Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.

Simmons Citrate Agar – Principle, Composition, Procedure, Result, uses

Simmons citrate agar test

Simmons Citrate Agar is a specialized solid microbiological culture medium which is used mainly to differentiate Gram-negative bacteria especially the members of Enterobacteriaceae family. It is used to test the ability of an organism to survive by using citrate as the sole carbon and energy source and ammonium salts as the sole nitrogen source. It … Read more

Gelatin Hydrolysis Test – Purpose, Principle, Procedure, Result.

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.

CAMP Test – Principle, Procedure, Result, Uses

Christie, Atkins, and Munch-Peterson (CAMP) test

CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.

Reverse CAMP test – Principle, Procedure, Result, Uses

Reverse CAMP test

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Biuret Test For Protein – Principle, Procedure, Result, Uses

Biuret Test For Protein Principle, Procedure, Result, Application.

Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.

Tollens’ Test – Principle, Procedure, Result, Application

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Oxidase Test – Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Isolation of Bacillus thuringiensis (Bt) from Soil sample

Procedure for Isolation of Bacillus thuringiensis (Bt) from Soil sample

Bacillus thuringiensis (Bt) is a Gram positive bacteria. It is a spore forming bacteria. It is found naturally in different environment like soil water dead insects and plant surface. It is known for producing special protein crystal during sporulation phase. These protein crystal is known as delta endotoxin (Cry and Cyt proteins). When these crystal … Read more

Nylander’s Test for Carbohydrates – Principle, Procedure, Result

Nylander’s Test for Carbohydrates Principle, Procedure, Result

Nylander’s Test is a chemical test. It is used for qualitative detection of reducing sugar mainly glucose in biological fluids like urine. It was developed in 1880s by Emil Nylander. It was used earlier as screening test for diabetes mellitus and other metabolic disorder. It is based on oxidation reduction reaction. In this test carbohydrate … Read more