Nelson-Somogyi method can be used to quantify the reduction of sugar using arsenolmolibdat and copper reagents. The principle behind the Nelson Somogyi method is the quantity of deposro oxide deposits which react with arsenomolibdate, which reduces to molybdine blue. The blue hue is determined by as absorbance. The keto and aldehyde free groups are regularly … Read more
The rapid furfural test is a type of chemical test which is used to determine the difference between fructose and glucose. The test for rapid furfural is like Molisch’s test however it utilizes concentrated hydrochloric acid instead of concentrated sulfuric acid, and the solution is then boiled. A dilute solution of sugar is added in … Read more
This is a test that is common for all carbohydrate larger than the tetroses. The test works on the basis that pentoses as well as hexoses can be dehydrated using conc. sulfuric acid, resulting in furfural or hydroxyl-methyl furfural or hydroxyl methyl furfural. These compounds condense with a-naphthol to create a purple condensation product. Molisch’s … Read more
Mucic acid test tests for lactose and galactose. The sugar is transformed into carboxylic acid using hot nitric acid. In order to produce dicarboxylic acids, aldoses are oxidized on each end of the open ring form. Chain fragmentation triggers ketoses to oxidize and produce a range of dicarboxylic acid. Galactraric acid (mucic acid) created by … Read more
Seliwanoff’s test is a type of chemical test that used to differentiate between ketose and aldose sugars. If the sugar has a ketone group, then it’s ketose. If a sugar has an aldehyde molecule, it is an aldose. This test is based on the idea that in the presence of heat, ketoses become more quickly … Read more
What is Bial’s test? Bial’s Test is a type of chemical test which is used to determine the existence of pentoses. It was named in honor of Manfred Bial, a German doctor. The ingredients are orcinol, hydrochloric acid and ferric chloride. If Pentose is present it will dehydrated to form furfural that is then reacted … Read more
The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.
Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.
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