Principle of Automated Cell Counter Types of Automated Cell Counters 1. Impedance-Based Cell Counters – This type of counter works on Coulter Principle, where the change in electrical resistance is measured when each cell passes by a small aperture. The cell suspension is flowed by electrolyte, and as the cells interrupt the current, the pulses … Read more
Principle of Cellulose Acetate Electrophoresis Materials Required for Cellulose Acetate Electrophoresis Steps / Procedure of Cellulose Acetate Electrophoresis Cellulose Acetate as Electrophoretic Support Matrix Application of Cellulose Acetate Electrophoresis Advantages of Cellulose Acetate Electrophoresis Limitation of Cellulose Acetate Electrophoresis Comparisons with Gel or Capillary Electrophoresis FAQ on Cellulose Acetate Electrophoresis
What is a Compound Microscope? 3D Model of Compound Microscope Working Principle of Compound Microscope Magnification Power of Compound Microscope The magnification power of compound microscope refers to ratio of size of final image to size of object seen by naked eye. It is obtained by multiplying magnification of objective lens and magnification of eyepiece … Read more
What is Pulsed-field Gel Electrophoresis (PFGE)? Pulsed Field Gel Electrophoresis (PFGE) is a method used for separation of very large DNA fragments by applying an electric field that periodically changes direction / switches among different electrodes, so that DNA pieces of differing sizes are reoriented and separated. (The technique allows resolution of DNA up to … Read more
Fluorescence Spectroscopy is an analytical method that studies the fluorescence emitted by a sample when it is excited by light (often UV or visible). Principle of Fluorescence Spectroscopy Applications of Fluorescence Spectroscopy in inorganic chemistry Special fluorimetric applications
What is a Water Bath? A Laboratory Water Bath can define as equipment used for maintaining constant temperature condition for samples during experiment or incubation process. It is basically consist of container filled with water, which heated to desired temperature and samples are placed inside for uniform heating purpose. The principle of operation based on … Read more
What is Laboratory Incubator? A Laboratory Incubator is described as a device that is used to grow and maintain microbial / cell cultures under controlled temperature, humidity, and gas conditions. The environment inside is usually kept stable, and temperature is controlled (often by thermostats/sensors), though fluctuations are caused by door openings. For mammalian cells, a … Read more
What is an autoclave? An autoclave is a device that is used for sterilization / disinfection by means of steam under pressure, and it is found in hospitals, labs, industry. Items are placed in a chamber where they are exposed to high-pressure saturated steam (commonly 121 °C for 15–20 minutes) so that microbes and spores … Read more
Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).
What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more
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