Anesthetic (MS222) Solution Preparation
Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.
Practical
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Carbenicillin Solution Preparation
Carbenicillin is an ampicillin analog with structure related to benzyl penicillin. It is effective against Gram-negative bacteria and exhibits limited activity against Gram-positive bacteria. Carbenicillin binds and inhibits the enzymes involved in the synthesis of bacterial cell wall. Since carbenicillin is less sensitive to β-lactamases than ampicillin, it is effective against ampicillin-resistant bacteria. It has … Read more
Solution and Cell/Culture/Growth Media Preparations
Chelation Medium Preparation To prepare 1L of Chelation Medium the following components are required. Component Amount Concentration NaCl (mw: 58.44 g/mol) 40.9 g 0.7 M MgCl2·6H2O (mw: 203.3 g/mol) 6.1 g 0.03 M MgSO4·7H2O (mw: 246.47 g/mol) 7.39 g 0.03 M KCl (mw: 74.55 g/mol) 1.49 g 0.02 M EGTA (mw: 380.35 g/mol) 7.6 g … Read more
Different Buffer preparation for Gel Electrophoresis
Acrylamide Stock Solution Preparation Application: Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains. To prepare 1L of Acrylamide Stock Solution the following components are required; Component Amount Concentration acrylamide (mw: 71.08 g/mol) 389.6 g 5.481 M bisacrylamide (mw: 154.17 g/mol) 10.4 … Read more
Preparation of Different pH Buffer
pH Buffer solution A pH buffer or hydrogen ion buffer is defined as an aqueous solution that is composed of a mixture of a weak acid and its conjugate base, or vice versa. In addition of a small amount of strong acid or base, it shows very little changes in pH. pH buffer is mainly … Read more
Physiological Buffer preparation
Tyrode’s Solution, Acidic Preparation To prepare 1L of Tyrode’s Solution, Acidic, the following components are required; Component Amount Concentration NaCl (mw: 58.44 g/mol) 8 g 0.1368 M KCl (mw: 74.55 g/mol) 200 mg 0.0027 M CaCl2•2H2O (mw: 147.01 g/mol) 240 mg 0.0016 M MgCl2•6H2O (mw: 203.3 g/mol) 100 mg 0.0005 M Glucose (mw: 180.16 g/mol) … Read more
Sample Preparation and composition
ACK Lysis Buffer Preparation ACK Lysis Buffer is used to lyse red blood cells. To prepare 1L of ACK Lysis Buffer the following components are required; Component Amount Concentration NH4Cl (mw: 53.49 g/mol) 8.02 g 0.15 M KHCO3 (mw: 100.12 g/mol) 1 g 0.01 M Na2EDTA (mw: 372.24 g/mol) 37 mg 0.0001 M ACK Lysis … Read more
Preparation of Crystal Violet for the Gram Stain
This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.
Tris-SDS Buffer Preparation of pH 6.8 and 8.8 for SDS PAGE
Tris-SDS Buffer (pH 6.8) is used to prepare buffer for stacking gel during SDS-PAGE (SDSPolyacrylamide gel electrophoresis). It is a discontinuous gel with a huge pores size to concentrate proteins prior to entering the separating zone. The buffer is sold in a 5X stock.
SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe
SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g 0.2 M EDTA (mw: 292.24 g/mol) 7.4 g 0.02 M SSPE (Blot, 20x, pH 7.4) Preparation Prepare 800 mL of distilled water in a suitable container. Add 175.3 g of Sodium chloride to the solution. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution. Add 7.4 g of EDTA to the solution. Add distilled water until the volume is 1 L. … Read more