17 min read 
Peptone Yeast Extract Broth Based Media are enriched nonselective media which include hemin and vitamin K to aid in the recovery of aggressive organisms like Prevotella Spp., Porphyromonas species, as well as the Bacteroides fragileis group.
19 min read 
MacConkey Sorbitol Agar is an adaptation of the formulation that was described by Rappaport and Henigh. It is selective and differential media for the detection of sorbitol-nonfermenting Escherichia coli serotype O157:H7 associated with hemorrhagic colitis. E. coli serotype O157:H7 is a human pathogen that is associated with hemorrhagic colitis which is caused by the actions of a Shiga-like toxins (SLT).
17 min read 
Thayer and Martin have reported improvement of the Chocolate Agar formulation that contained antimicrobics vancomycin and colistin, Nystatin. These ingredients were added to limit the growth of contaminants, inhibit the development of the saprophytic Neisseria sp. and to enhance the development of the pathogenic Neisseria. Martin and Lester have added an antibiotic, trimethoprim, which made them more selective. They also demonstrated to be effective in the fight against Proteus spp. Trimethoprim lactate refrains Proteus swarming. The resultant medium is known as Modified Thayer Martin. It’s a suitable and rich medium to isolate as well as cultivation of the Neisseria species. from mixed flora and suppression of other Gram-negative diplococci and gram-negative bacilli yeast and other gram-positive organisms.
14 min read 
Roantree et al introduced a medium for the isolation of streptococci from group A beta-hemolytic. The medium was enriched with yeast nucleic acids and maltose boosted the size of colonies and improved the clarity, sharpness and precision of the hemolytic zones created by these organisms.
13 min read 
MRS Broth was developed by deMan, and co. in order to facilitate the most abundant growth of lactobacilli derived from feces, oral and other samples. Lactobacilli MRS Broth is a better media for lactobacilli. It allows for healthy growth and is especially suitable for several very discerning strains that grow poorly in other media.
14 min read 
Cystine tryptic agar (CTA) which is also referred to as cystine trypticase agar was created by Vera as an semi-solid growth medium to identify and maintain the health of microorganisms for long time period of time when stored at suitable temperatures. It’s used for determination of the motility of bacteria and, when combined with carbohydrate, is involved in the fermentation process and the differentiation of microorganisms that are fastidious, such as Neisseria and streptococci, as well as pneumococci and non-spore-forming anaerobes.
18 min read 
Raymond Sabouraud created Sabouraud Dextrose Aga or SDA in 1892. Sabouraud Dextrose Aggar is useful in the cultivation of fungi (yeasts and moulds), especially for skin infections.
4 min read 
To ensure that clinical specimens are properly cultured, two types of media are required. The non-selective medium (e.g. Brain Heart Infusion Agar) should be used. Other media should be specific and tailored to isolate pathogenic fungi.
4 min read 
A selective medium called phenylethyl alcohol (PEA), is used to cultivate Gram positive organisms, especially cocci, in a sample containing pathogens. Phenylethyl alcohol is the active ingredient that inhibits or significantly reduces growth of Gram-negative organisms.
2 min read 
Anaerobic Culture Media used in anaerobic bacteriology may be prepared in a fresh manner or bought from commercial vendors.
Media for anaerobic fermentation that is created in the laboratory should be used within two weeks after the time of preparation, as prolonged storage can degrade the quality of the medium due to peroxide accumulation and dehydration.