Chocolate Agar Media – Principle, Composition, Preparation, Result
Chocolate Agar (CAP or CHOC) is a nonselective enhanced medium that is used to identify and isolate of pathogens that are fastidious.
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Xylose Lysine Deoxycholate (XLD) Agar – Composition, Preparation, Principle, Uses
Xylose Lysine Deoxycholate agar (XLD) is a selective medium that allows for the isolation and growth of Salmonella and Shigella species using clinical samples or food. Taylor developed XLD Agar to aid in the differentiation, isolation and identification of enteric disease agents and support the growth more specialized enteric organisms. XLD Agar is a well-proven medium that allows the growth of Shigella species. It is also an excellent medium to isolate Salmonella species. It has a pH value of 7.4, giving it a bright pink to red color due to the indicator phenol.
Mannitol Salt Agar (MSA) – Composition, Principle, Preparation, Results, Uses
Mannitol Salt Agar (MSA) is utilized as a differential and selective medium to isolate and detect Staphylococcus aureus in clinical and non-clinical samples. It is recommended for identification and enumeration of coagulase positive Staphylococci that are present in milk, food as well as other specimens. MSA also promotes an increase in the number of particular group of specific bacteria, while limiting the growth of other bacteria.
New York City Agar – Composition, Preparation, Result, Uses
NYC Agar Base medium was initially created by Fauer, Weisburd and Wilson at the New York City Department of Health to isolate the pathogenic Neisseria strains from human samples. NYC medium, designed primarily to isolate pathogenic Neisseria is also able to support the growth of mycoplasmas with large colonies or mycoplasmas T (Ureaplasma). It is a translucent medium, highly selective, allowing direct microscopic observations and presumed identification of mycoplasmas and Neisseria gonorrhoeae.
Martin Lewis Agar – Composition, Preparation, Principle, Uses
What is Thayer Martin Medium? Composition of Martin Lewis Agar Ingredients Gms/L Casein Peptone 7.5 Meat Peptone 7.5 Corn Starch 1.0 Potassium Phosphate, dibasic 4.0 Potassium Phosphate, monobasic 1.0 Sodium Chloride 5.0 Agar 10.0 Hemoglobin 10.0 Dextrose 1.5 Enrichment Bio-X 10 ml Antibiotics V.C.A.T 10 ml pH 7.2 +/- 0.2 at 25º C Enrichment Bio-X … Read more
Peptone Water – Principle, Composition, Preparation, Uses
Peptone Water is a type of broth medium. This media is mainly used to grow the organism and is also used as a base for determining carbohydrate fermentation patterns of non-fastidious organisms. It is also used to detect indole production by the organism.
Alkaline Peptone Water – Preparation, Composition, Principle, Application.
Clinical materials containing small numbers of Vibrio should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar (M189). Alkaline Peptone Water is a suitable enrichment broth for this purpose.
Blood Agar – Preparation, Composition, Application, and limitation
production of blood agar after the addition of liquid blood.
Sheep blood agar – Composition, Principle, Procedure, Uses.
Sheep blood agar The principle of sheep blood agar Haemolysins are exotoxins created by bacteria that kill Red blood cell clots. The haemolytic process can be observed on blood Agar Plates. In blood agar plates, colonies of haemolytic bacterium may be covered by a the clear, uncoloured zone, in which the Red blood cells are … Read more
Mueller Hinton Agar (MHA) Composition, Principle, Preparation
What is Mueller Hinton Agar (MHA)? Why MHA is used for antibiotic susceptibility testing (AST)? Mueller Hinton Agar (MHA) is commonly used for antibiotic susceptibility testing due to several key reasons: Mueller Hinton Agar (MHA) Principle The principle of Mueller Hinton Agar (MHA) revolves around its composition and the controlled levels of various components to … Read more