What is Reversed-Phase Chromatography? Principle of Reversed-Phase Chromatography The matrix in reversed-phase chromatography Stationary phases Mobile phases Protocol of Reverse-phase chromatography Uses of Reverse-phase chromatography Advantages of Reverse-phase chromatography Limitations of Reverse-phase chromatography FAQ
What is Liquid Chromatography? Types of Liquid Chromatography 1. Normal Phase Liquid Chromatography– This type was characterized by polar stationary phase and non-polar mobile phase, separation was achieved mainly by adsorption and polarity differences. Silica gel and alumina are often used as stationary matrix, while solvents like hexane, chloroform are used as mobile phase (sometimes … Read more
What is Hydrophobic Interaction Chromatography (HIC)? Principle of Hydrophobic Interaction Chromatography (HIC) Protocol of Hydrophobic Interaction Chromatography (HIC) Factors that Affect Hydrophobic Interaction Chromatography (HIC) Uses of Hydrophobic interaction chromatography Advantages of Hydrophobic interaction chromatography Limitations of Hydrophobic interaction chromatography FAQ
What is Flash chromatography? Principle of Flash column chromatography Flash column chromatography equipment Flash chromatography General consist of following parts 1. Pump Systems Type of pump There are different types of pumps commonly used in flash chromatography systems, each offering specific capabilities and functionality. Here are some notable types of pumps: The selection of a … Read more
What is Chromatography? Chromatography Definition Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description Gas … Read more
What is Two-Dimensional (Crossed) lmmunoelectrophoresis? Principle of Two-Dimensional lmmunoelectrophoresis The principle of two-dimensional (crossed) immunoelectrophoresis (2-D IEP) involves a two-step electrophoretic separation that permits antigen mixtures to be analysed and quantitated. In the first dimension the antigen (protein) mixture is loaded on an agarose gel and it is separated by electrophoresis by charge and mobility … Read more
What is Immunoelectrophoresis? Immunoelectrophoresis is defined as the separation of proteins by electrophoresis followed by their identification through immunologic (antigen-antibody) reactions. Immunoelectrophoresis Definition Immunoelectrophoresis is a biochemical technique that combines electrophoresis and antibody-antigen reactions to separate and identify proteins based on their antigenic properties. Objectives of Immunoelectrophoresis Principle of Immunoelectrophoresis The method is based on … Read more
What is Polyacrylamide Gel Electrophoresis (PAGE)? Definition of Polyacrylamide Gel Electrophoresis (PAGE) Polyacrylamide Gel Electrophoresis (PAGE) is a technique used to separate and analyze proteins or nucleic acids based on their mobility in a porous polyacrylamide gel matrix under the influence of an electric field. Types of Polyacrylamide Gel Electrophoresis (PAGE) Native PAGE – Proteins … Read more
What is SDS-PAGE? Definition of SDS-PAGE SDS-PAGE is a technique used to separate proteins based on their molecular weight by using a gel matrix and the denaturing agent sodium dodecyl sulfate (SDS). Objective Principle of SDS-PAGE SDS–PAGE refers as Sodium Dodecyl Sulfate–PolyAcrylamide Gel Electrophoresis, and its main principle based on separation of proteins mainly by … Read more
What is Mass Spectrometry (MS)? Definition of Mass Spectrometry (MS) Mass Spectrometry (MS) is an analytical technique used to identify the chemical composition of a sample by measuring the mass-to-charge ratio of its ions, resulting in a mass spectrum that reveals the molecular and structural information of the sample. Principle of Mass Spectrometry (MS) Working of Mass … Read more
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