Join Our Whatsapp and Telegram Channel to Get Free eBooks Telegram | Whatsapp

MIC Test Strip – Quantitative assay for determining the Minimum Inhibitory Concentration

MIC Test Strip

  • The Minimum Inhibitory Concentration (M.I.C.) of antimicrobial drugs against bacteria and the detection of resistance mechanisms can be found with the help of a MIC Test Strip, a quantitative assay.
  • Paper strips with predetermined antibiotic concentration gradients are known as minimum inhibitory concentration (MIC) strips (covering 15 double dilutions as for conventional MIC methods).
  • The MIC Test Strip is a customised porous strip (International Patent) impregnated with an antibiotic concentration gradient through 15 two-fold dilutions of the standard M.I.C. method.
  • The antimicrobial agent and its MIC (minimum inhibitory concentration) are both labelled on one side of the strip.
  • The double-sided gradient incorporates the required diagnostic reagents for detecting ESBL, MBL, GRD, AmpC, and KPC.
  • MIC Test Strips come in a wide variety of styles. We offer 10, 30, and 100 test runs of each setup.
MIC Test Strip
MIC Test Strip | Image Source: microbiologie-clinique.com

Principle

  • The preformed exponential gradient of antimicrobial agent is transferred instantly to the agar matrix upon application of the MIC Test Strip onto an inoculated agar surface. 
  • A symmetrical inhibition ellipse, with its centre along the strip, forms after incubation for at least 18 hours. 
  • At the point where the edge of the inhibition ellipse meets the strip MIC Test Strip, the MIC is read straight from the scale in terms of g/mL. 
  • For resistance detection approaches, other growth/inhibition patterns may also be noticed.
MIC Test Strip
MIC Test Strip | Image Source: microbiologie-clinique.com

Procedure

  1. To get a turbidity of 0.5 McFarland, lift well-isolated colonies from an agar plate (con fl uent or almost con fluent growth should be obtained after incubation).
  2. Dip a sterile swab into the inoculum suspension and squeeze it against the side of the test tube to get rid of any extra fluid.
  3. Before putting on the CMI strips, give the surface 15 to 20 minutes to soak up any extra moisture so that it is completely dry.
  4. Apply the strip to the agar surface with the scale facing up and the strip code facing the outside of the plate. Press the strip onto the agar surface with sterile forceps and make sure that the entire length of the antibiotic gradient is in full contact with the agar surface. Once the tape is on, you shouldn’t move it.
  5. If there are air bubbles under the strip, use sterile forceps to move them to the edge, being careful not to move the strip over the agar. Start with the least concentrated solution and work your way up to the most concentrated one.
  6. Incubate the agar plates at 35 2 °C while they are upside down.
  7. After 18 hours or more of incubation, a symmetrical inhibition ellipse forms in the middle of the strip. At the point where the edge of the inhibition ellipse meets the MIC test strip, the MIC is read directly from the g/mL scale.

Reading MIC strips

After incubation for at least 18 hours, a symmetrical inhibition ellipse forms in the centre of the strip. At the point when the edge of the inhibition ellipse meets the MIC test strip, the MIC is read straight from the g / mL scale.

MIC Test Strip
MIC Test Strip | Image Source: http://www.liofilchem.net/en/pdf/mic_brochure.pdf
MIC Test Strip
MIC Test Strip | Image Source: http://www.liofilchem.net/en/pdf/mic_brochure.pdf

References

  • https://www.fishersci.com/us/en/browse/90222067/mic-test-strips?page=1#:~:text=Learn%20More-,MIC%20Test%20Strips%20are%20a%20quantitative%20assay%20for%20determining%20the,Ticarcillin%2DClavulanic%20Acid
  • https://www.liofilchem.com/featured-products/mic-test-strip.html
  • https://www.liofilchem.com/solutions/clinical/arm/mts-mic-test-strip
  • http://www.liofilchem.net/en/pdf/mic_brochure.pdf
  • https://www.launchdiagnostics.com/product/liofilchem-mic-test-strip-mts/
  • https://www.rapidmicrobiology.com/products/mic-test-strip-2
  • https://www.rapidmicrobiology.com/news/new-liofilchem-mic-test-strips
  • https://bestbion.com/wp-content/uploads/2017/11/01-0001-LL-MIC-Test-Strip_small.pdf
  • https://microbiologie-clinique.com/mic-test-strip.html

Related Posts

Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.

A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?
A new weapon in the battle against antibiotic resistance 16 Important Skills Needed For A Successful Career in Bioinformatics Top 5 High-Paying Biotech Jobs in India (No PhD Required) Top Emerging Trends in Bioinformatics Important Skills Needed For A Successful Career in Bioinformatics Research reveals plant pathogens repurpose phage elements for bacterial warfare Scientists show the key role of spleen and extracellular vesicles in cryptic malaria infections Scientists reveal molecular link between glucose sensing and pyroptosis cell death Scientists reconstruct ancient genomes of the two most deadly malaria parasites to identify origin and spread What are TaqMan probes?

Adblocker detected! Please consider reading this notice.

We've detected that you are using AdBlock Plus or some other adblocking software which is preventing the page from fully loading.

We don't have any banner, Flash, animation, obnoxious sound, or popup ad. We do not implement these annoying types of ads!

We need money to operate the site, and almost all of it comes from our online advertising.

Please add biologynotesonline.com to your ad blocking whitelist or disable your adblocking software.

×