Practical

What is Native Polyacrylamide Gel Electrophoresis? Principle of Native Polyacrylamide Gel Electrophoresis (PAGE) The principle of Native Polyacrylamide Gel Electrophoresis (PAGE) is based on the separation of proteins according to their net charge, size, and shape in their native structure. The technique takes advantage of the fact that most proteins carry a net negative charge … Read more

Microbiology laboratories require well-built rooms that are equipped with tools, glassware and equipment. Test tubes, culture tubes, Petri dishes and Erlenmeyer flasks are the most important types of glassware in a microbiological lab. 1. Test tube, culture tube and screw-capped tubes 2. Petri dish 3. Pipette 4. Pasteur pipette 5. Erlenmeyer flasks 6. Volumetric flasks … Read more

McIntosh and Fildes’s anaerobic jar is a tool utilized in microbiology laboratories, to generate anaerobic circumstances (anaerobiosis) to cultivate obligate anaerobes, such as Clostridium spp. Anaerobiosis derived from McIntosh and Fildes anaerobic jar is among the most effective and most frequently employed methods for anaerobiosis however it requires expensive special equipment and a vacuum pump. … Read more

GasPak anaerobic system is utilized to create an oxygen-free atmosphere to encourage the growth of microorganisms that live in anaerobic environments. In the present, it is the most frequently utilized method for anaerobiosis. It is easy to do and works well for laboratories with a smaller amount of samples. Components of GasPak Anaerobic System Principle … Read more

What is BOD Incubator? BOD incubator Definition The BOD (Biological Oxygen Demand) Incubator is a specialized laboratory instrument designed to provide a controlled temperature environment, typically around 20 degrees centigrade, for microbial and biochemical studies, particularly for assessing the oxygen demand of microorganisms in water samples. BOD incubator Principle Components of BOD Incubator The BOD … Read more

Laboratory incubators are essential equipment in research laboratories, providing a controlled environment for various experiments and cell culture processes. They are designed to maintain precise temperature, humidity, and atmospheric conditions within an enclosed chamber, enabling the optimal growth and development of microorganisms, cell lines, and other biological samples. These incubators play a crucial role in … Read more

Differential centrifugation (also called the differential velocity method) is a common process in the fields of biochemistry and cell biology it is utilized to differentiate organelles and other subcellular particles by their sedimentation rates. Although it is commonly used in biochemical analysis it is a general method that is useful for the crude elimination of … Read more

Autoclaving is one method that uses steam to sterilize. Autoclave bags are utilized for sterilization at high temperatures to keep low-temperature plastics in the autoclave bags from sticking against the side of the sterilizer or obstructing the plumbing for the sterilizer’s vent. Autoclaves function by using a combination of pressure, steam, and time. They work … Read more

Density Gradient Centrifugation Density gradient centrifugation refers to an approach to separation between molecules, where the separation is determined by the density of the molecules when they travel through a gradient under the force of centrifugal. Principle of Density gradient centrifugation Density gradient centrifugation relies on the premise that molecules settle under the force of … Read more