Soil contain different types of microorganisms which are listed below; Principle of Isolation of Microorganisms from Soil During this process, the soil sample is collected from the study site. After that, the soil sample is dissolved in distilled water and then serial dilution is performed. Then the spread-plate/streak plate method is performed on the sample … Read more
Making a Wet Mount Staining a Slide (After Making a Wet Mount) Adjusting Focusing Tension There is a ring on the inside portion of the right-hand focusing knob on all other compound microscope versions (490 and above). This ring can be manually adjusted to increase or decrease tension. Changing the Eyepiece (M Series Microscope) A … Read more
What is Centrifuge? What is Centrifuge Balancing? Why it is important to balance a centrifuge? – Why is centrifuge balanced? What Happens if a Centrifuge is Unbalanced? How Centrifuge Works? What is Centrifuge Tube? How to balance in different centrifuge? If you are utilising a portable benchtop instrument, you must ensure that the centrifuge is … Read more
What is a serial dilution? Serial dilution definition Serial dilution is a methodical process in which a solution is diluted sequentially to produce a series of progressively reduced concentrations. What is the purpose of serial dilution? – Serial Dilution Objectives Serial dilution serves as a foundational procedure in numerous scientific disciplines, especially microbiology. The primary … Read more
Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.
It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).
Of all the options to sterilize (killing or eliminating all microorganisms, which includes the spores of bacterial growth) moist heat that is saturated steam with pressure has been the most frequently utilized and most reliable method. Moisture has more penetrating power than dry heat, and at a certain temperature, causes a greater reduction in the quantity of live microorganisms. Steam sterilization is safe cheap, low-cost, quickly microbicidal, as well as sporicidal. It quickly heats and penetrates into fabrics.
Because the aim of biologists is to cultivate microorganisms or eukaryotic cells with no introduction of any extraneous organisms aseptic methods are essential for ensuring that experiments are conducted with precision and accuracy. Always keep in mind that a totally safe working environment for scientists does not exist.
Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more
The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more
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