Preparation of Crystal Violet for the Gram Stain
This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.
Practical
Subcategories
Tris-SDS Buffer Preparation of pH 6.8 and 8.8 for SDS PAGE
Tris-SDS Buffer (pH 6.8) is used to prepare buffer for stacking gel during SDS-PAGE (SDSPolyacrylamide gel electrophoresis). It is a discontinuous gel with a huge pores size to concentrate proteins prior to entering the separating zone. The buffer is sold in a 5X stock.
SSC (Northern Blot, 20x) and SSPE (Blot, 20x, pH 7.4) Preparation and Recipe
SSPE (Blot, 20x, pH 7.4) Preparation and Recipe Required components Component Amount Concentration Sodium chloride (mw: 58.44 g/mol) 175.3 g 3 M Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) 27.6 g 0.2 M EDTA (mw: 292.24 g/mol) 7.4 g 0.02 M SSPE (Blot, 20x, pH 7.4) Preparation Prepare 800 mL of distilled water in a suitable container. Add 175.3 g of Sodium chloride to the solution. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution. Add 7.4 g of EDTA to the solution. Add distilled water until the volume is 1 L. … Read more
Calcium Chloride Stock Solution Preparation
Calcium Chloride Stock Solution Preparation Calcium chloride is a common calcium salt used in a variety of applications. To prepare 1L of Calcium Chloride Stock Solution the following components are required. Component Amount Concentration CaCl2 (mw: 110.98 g/mol) 58 g 0.5226 M Preparation procedure Prepare 800 mL of distilled water in a suitable container. Add … Read more
Bath Solution (Ca2+/Mg2+ Free) Preparation
Bath Solution Preparation To prepare 1L of Bath Solution (Ca2+/Mg2+ Free) the following components are required; Component Amount Concentration KOH (mw: 56.11 g/mol) 7.854 g 0.14 M HEPES (mw: 238.3 g/mol) 4.766 g 0.02 M KCl (mw: 74.55 g/mol) 149 mg 0.002 M EGTA (mw: 380.35 g/mol) 1.902 g 0.005 M Methanesulfonic acid (mw: 96.11 … Read more
Pyronin Y (PY) Solution Preparation and Application
Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.
Nuclear Fast Red Solution Preparation and Application
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used as a reagent for calcium in tissue where it forms a scarlet lake.
Mayer’s Hemalum Solution Preparation and Application
This “Mayer’s hemalum solution – for microscopy” is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin.
Immunofluorescence Quenching Solution Preparation and Application
Immunofluorescence Quenching Solution is used in immunofluorescence and immunocytochemistry applications.
Cresol Red Solution Preparation and Uses
Cresol red is used as a tracking dye for DNA/RNA in agarose gel electrophoresis and protein in polyacrylamide gel electrophoresis.