What is Gel Permeation Chromatography? Gel Permeation Chromatography Principle The separation of components based on their molecular weight or size is the basis of gel permeation chromatography (GPC), also known as gel filtration or size exclusion chromatography. The method employs a stationary phase composed of a porous polymer matrix. The solvent that serves as the … Read more
What is Centrifugal force? Definition of Centrifugal force Centrifugal force is an outward fictitious force experienced by objects moving in a circular path, directed away from the center of rotation. Centrifugal force formula The centrifugal force can be calculated using different formulas depending on the available information. If the velocity (v) of the moving object … Read more
An Automated Cell Counter is a machine or device that is used to count the number of cells in a biological sample. It uses various technologies such as image analysis, impedance measurement, or fluorescence to accurately determine the number of cells present. The importance of cell counting lies in its widespread applications in the fields … Read more
Cellulose Acetate Electrophoresis (CAE) is a method of separating charged molecules, including proteins, based on their charge and size. It uses a cellulose acetate sheet as a medium for separation, and an electrical current to move the molecules through the medium. The charged molecules are separated based on their migration rate through the cellulose acetate … Read more
A microtome is a scientific instrument used to cut very thin slices of material, typically for use in microscopy. The material being sliced can be a variety of things, including biological tissue, plant specimens, or materials for industrial or electronic purposes. The microtome works by holding the material in place and then moving a blade … Read more
A compound microscope is a class of optical or light microscope. I have already discussed about basics of a microscope on my previous note “Parts of Microscope with their Functions and Working Principle”, you can check them to get an idea about from which class compound microscope belongs and what is a microscope, and more. … Read more
What is Pulsed-field Gel Electrophoresis (PFGE)? Principle of Pulsed Field Gel Electrophoresis (PFGE) The principle of pulsed-field gel electrophoresis (PFGE) revolves around the separation of DNA fragments based on their size using an electric field. In conventional gel electrophoresis, small DNA fragments migrate more easily through the gel matrix than large fragments. However, when dealing … Read more
Fluorescence spectroscopy is an analytical technique used to examine the characteristics and interactions of molecules. It is founded on the idea that when a molecule absorbs a photon of light and becomes excited, it emits light at a longer wavelength, a phenomenon known as fluorescence. This emitted light can be collected and studied in order … Read more
The process of collecting bacteria from the air for analysis involves several techniques, one of the simplest being the utilization of open Petri dishes filled with a growth medium. These dishes are exposed to the air, allowing airborne bacteria to settle and subsequently grow on the medium, which can then be examined to identify the … Read more
Exploring the microscopic world within soil requires precise techniques for the effective sampling of microorganisms, such as bacteria. These organisms play crucial roles in various ecological processes, including nutrient cycling, and understanding their presence and diversity is vital for both scientific research and environmental monitoring. Two widely recognized methods facilitate the direct observation of these … Read more
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