Cell viability analysis using Propidium Iodide is a simple method in which the dye is used to distinguish between living and dead cells. It is the process where Propidium Iodide (PI) is added to a cell suspension and the stained cells is then examined usually by flow cytometer. PI is a red-fluorescent dye that binds … Read more
Loeffler’s methylene blue staining is a simple staining method used mainly for observing the morphology of Corynebacterium diphtheriae. It is the process where Loeffler’s alkaline methylene blue solution is applied on a fixed smear, and this stain contains a small amount of potassium hydroxide (KOH) which makes the solution alkaline. It is the alkalinity that … Read more
Giemsa stain is a polychromatic nucleic acid stain that is used mainly for demonstrating different blood cells and parasites in stained blood films. It is named after the German chemist Gustav Giemsa. It belongs to a group of stains known as Romanowsky stains. These are neutral stains made from a mixture of oxidized methylene blue, … Read more
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
There are two methods which are commonly used to stain bacterial endospore such as Schaeffer Fulton and Klein methods.
Toluidine blue is a basic thiazine metachromatic dye that stains nuclei blue, and can be used to differentiate different types of granules (e.g. within mast cells).
Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
Kinyoun method also known as Kinyoun stain (cold method), was first developed by Joseph J. Kinyoun.
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