Millon’s Test -Principle, Procedure, Result, Uses
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
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Bial’s Test- Principle, Procedure, Result, Uses
Bial test is a biochemical test which is used for the detection of pentose sugars and to differentiate them from hexose sugars. It is mainly employed for identifying sugars like ribose and xylose and is also useful for the detection of RNA because RNA contains ribose sugar. This test is based on the reaction of … Read more
Beta (β) Lactamase Test – Principle, Procedure, Results
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
Nitrocefin Test (Beta-Lactamase Test) – Principle, Procedure, Uses
The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.
Mucic acid test – Principle, Procedure, Result, Uses
Mucic acid test is a specific chemical test used for the identification of galactose and galactose containing sugars like lactose. It is also referred to as galactaric acid test. It is based on the oxidation reaction of sugars with concentrated nitric acid. In this test the sugar sample is heated with concentrated nitric acid which … Read more
Molisch Test – Objective, Principle, Procedure, Result
This is a test that is common for all carbohydrate larger than the tetroses. The test works on the basis that pentoses as well as hexoses can be dehydrated using conc. sulfuric acid, resulting in furfural or hydroxyl-methyl furfural or hydroxyl methyl furfural. These compounds condense with a-naphthol to create a purple condensation product. Molisch’s … Read more
Rapid Furfural Test for Glucose and Fructose – Principle, Procedure, Result
Rapid Furfural Test is a qualitative chemical test used to differentiate ketohexoses from aldohexoses based on the rate of dehydration reaction. It is mainly used for the identification of fructose and other ketose sugars in the presence of aldose sugars like glucose. It is based on the principle that ketohexoses are dehydrated rapidly when heated … Read more
Fehling’s Test – Principle, Procedure, Result
This test also can differentiate between ketone functional groups and water-soluble carbohydrates.
Solubility Tests of Proteins – Principle, Procedure, Result, Uses
Solubility Tests is a type of biochemical test which is performed to check the ability of compounds to dissolve within a liquid solvent.
Alkaline Phosphatase Test of Milk – Principle, Procedure, Result, Uses
Alkaline Phosphatase (ALP) is an enzyme that is naturally found within all the raw milks that is utilized to determine the quality of pasteurization of milk. A complete pasteurization process can deactivate the enzyme below levels that can be detected by traditional methods. Because the stability of the heat of ALP is higher than the stability of pathogens that could be found in milk, it serves as a metric of security. However, failure for detection of ALP activity is not a mean an item is safe from pathogens.