What is Salmonella? Salmonella is a group of gram-negative bacillus bacteria that is responsible for causing food poisoning and subsequent infections in the intestinal tract. While some infections can be effectively treated, there are certain strains that have developed resistance to antibiotics, making them potentially deadly. Consequently, Salmonella infections should not be underestimated and require … Read more
E. coli, short for Escherichia coli, is a versatile bacterium that can be found in a variety of environments, including certain foods, soil, and the intestines of animals, including humans. It belongs to the genus Escherichia and exhibits a wide diversity of strains. While many strains of E. coli are harmless and even beneficial, some … Read more
What is Kirby Bauer Disk Diffusion test? The Kirby-Bauer disk diffusion test is a laboratory method used to determine the effectiveness of antibiotics against bacterial strains. The test is named after its inventors, Drs. Paul Kirby and Robert Bauer, who developed it in the 1960s. In the Kirby-Bauer test, a bacterial culture is spread evenly … Read more
Antimicrobial Susceptibility Testing (AST) Techniques of Antimicrobial Susceptibility test Antimicrobial susceptibility tests in the laboratory can be done by using: Dilution susceptibility tests Disc diffusion susceptibility tests Kirby-Bauer NCCLS modified disc diffusion technique Stokes disc diffusion technique Disadvantages of antimicrobial susceptibility tests Susceptibility tests determine the antimicrobial effect against bacteria in conditions of laboratory (in … Read more
What is Dark Field Microscopy? Definition of Dark Field Microscope A darkfield microscope is a type of optical microscope that uses oblique illumination to light the specimen, causing it to appear bright against a dark background. This technique enhances the visibility of transparent and living specimens by excluding the unscattered light from the image. Principle … Read more
The concept of using fluorescence to study biological materials dates back to the late 19th century, but the first practical fluorescence microscope was not developed until the 1930s. In 1938, Robert Goldstein and Richard Manly, researchers at the University of California, Berkeley, published a paper describing the use of fluorescence to study cells and tissues … Read more
What is Diaphragm of a Microscope? History of Diaphragm Definition of Diaphragm of a Microscope The diaphragm of a microscope is a component that controls the amount of light that passes through the specimen being observed. It is typically located between the light source and the stage, and consists of a series of adjustable blades … Read more
UV-Vis Spectroscopy, short for Ultraviolet-Visible Spectroscopy, is an analytical technique widely used in the fields of chemistry, physics, biochemistry, and molecular biology. It’s based on the absorption or transmission of UV and visible light by substances. At its core, UV-Vis Spectroscopy involves shining ultraviolet or visible light through a sample and measuring how much of … Read more
UV Spectroscopy is a scientific method that’s like a detective tool for scientists. It helps them understand what’s happening in a molecule when it interacts with light, specifically ultraviolet light. This type of light is invisible to our eyes but plays a crucial role in the world of molecules. Imagine a molecule as a tiny, … Read more
What is Objective Lens? Purpose of Objective Lenses Objective lenses serve several key purposes in optical instruments, playing a critical role in magnification, image formation, and resolution. The primary purposes of objective lenses include: Specification of Objective Lens The specification of an objective lens encompasses several key parameters that define its performance and suitability for … Read more
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