Optochin Susceptibility Test – Principle, Objective, Procedure And Results

Optochin Susceptibility Test - Principle, Objective, Procedure And Results

Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides. 

Ninhydrin Test Principle, Procedure, Result, Uses

Ninhydrin Test Principle, Procedure, Result, Uses

What is the Ninhydrin Test? The ninhydrin chemical test is used for determining whether an analyte contains any amines or amino acids. In this test, ninhydrin (a chemical compound with the formula C9H6O4; IUPAC name: 2,2-dihydroxyindane-1,3-dione) is added to a test solution of the analyte. The formation of a deep blue color within the test … Read more

Phenylalanine Deaminase Test Principle, Procedure, Result

Phenylalanine Deaminase Test Principle, Procedure, Result

Objective of Phenylalanine Deaminase Test Principle of Phenylalanine Deaminase Test Requirements For the test Composition of Phenylalanine agar DL-Phenylalanine: 2 gm Yeast extract: 3 gm Sodium chloride: 5 gm Disodium phosphate: 1 gm Agar: 12 gm Distilled water: 1000 ml pH: 7.3 Preparation of Phenylalanine agar slant Note: Following autoclaving, the medium should appear lighter … Read more

Peroxide Value Test Principle, Procedure, Result

Peroxide Value Test Principle, Procedure, Result

Unsaturated fish oils are especially vulnerable to oxidation, resulting in peroxides when stored in cold or freezing conditions for storage. Peroxides are precursors to breakdown products that can cause rancid taste in fat. Peroxide levels are an indicator of oxidation in the initial stages of lipid degradation. The index is less reliable in the latter … Read more

Picric Acid Test for Glucose – Principle, Procedure, Result

Picric Acid Test for Glucose - Principle, Procedure, Result

Picric acid test is used for reducing sugars, and is a highly sensitive chemical tests. Picric acid reactions (toxic yellow crystal solid) which is also referred to chemically as 2,4 6-trinitrophenol (TNP) reacts with sugars that are reduced to create Picramic Acid that is red-colored. The Picric Acid test is made up of a sugar … Read more

Nelson Somogyi Method for Determination of reducing sugars

Nelson Somogyi Method for Determination of reducing sugars

Nelson-Somogyi method can be used to quantify the reduction of sugar using arsenolmolibdat and copper reagents. The principle behind the Nelson Somogyi method is the quantity of deposro oxide deposits which react with arsenomolibdate, which reduces to molybdine blue. The blue hue is determined by as absorbance. The keto and aldehyde free groups are regularly … Read more

Rapid Furfural Test for Glucose and Fructose

Rapid Furfural Test for Glucose and Fructose

The rapid furfural test is a type of chemical test which is used to determine the difference between fructose and glucose. The test for rapid furfural is like Molisch’s test however it utilizes concentrated hydrochloric acid instead of concentrated sulfuric acid, and the solution is then boiled. A dilute solution of sugar is added in … Read more

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