Reverse CAMP Test: Principle, Procedure, Results and Uses
Learn the reverse CAMP test principle, procedure and result, including bow-tie hemolysis in Clostridium perfringens, positive organisms, uses and CAMP test differences.
Biology Practicals
Biuret Test For Protein – Principle, Procedure, Result, Uses
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.
Tollens’ Test – Principle, Procedure, Result, Application
It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.
Oxidase Test – Principle, Procedure, Result, Application
The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .
Isolation of Bacillus thuringiensis (Bt) from Soil sample
Bacillus thuringiensis (Bt) is a Gram positive bacteria. It is a spore forming bacteria. It is found naturally in different environment like soil water dead insects and plant surface. It is known for producing special protein crystal during sporulation phase. These protein crystal is known as delta endotoxin (Cry and Cyt proteins). When these crystal … Read more
Nylander’s Test for Carbohydrates – Principle, Procedure, Result
Nylander’s Test is a chemical test. It is used for qualitative detection of reducing sugar mainly glucose in biological fluids like urine. It was developed in 1880s by Emil Nylander. It was used earlier as screening test for diabetes mellitus and other metabolic disorder. It is based on oxidation reduction reaction. In this test carbohydrate … Read more
Picric Acid Test for Reducing Sugars – Principle, Procedure, Result and Uses
Picric Acid Test is a qualitative chemical test used for the detection of reducing sugars in a given sample. It detects sugars which can act as reducing agents under alkaline condition. The test is based on the reducing property of these sugars. In alkaline medium, the reducing sugar reacts with yellow picric acid (2,4,6-trinitrophenol). During … Read more
Lane-Eynon Method – Principle, Procedure, Result, Uses
The Lane–Eynon method is a volumetric titration technique used for the quantitative estimation of reducing sugars in food and dairy products. It is commonly used for the determination of sugars such as dextrose, maltose, and lactose. This method is based on the reduction of Fehling’s solution by reducing sugars under boiling conditions. It is simple, … Read more
Gerhardt’s test – Principle, Procedure, Result
Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.
Seliwanoff’s Test – Principle, Procedure, Result, Uses
Seliwanoff’s Test is a biochemical colour reaction that is used mainly to distinguish between ketose sugar and aldose sugar. It is performed by using Seliwanoff’s reagent (resorcinol + concentrated hydrochloric acid) and the sugar solution is heated with this reagent. In this test the acid act as dehydrating agent and the reaction is based on … Read more