Membrane filtration method is a laboratory method which is used for estimation of microbial load (bioburden) in liquid sample like drinking water environmental water and pharmaceutical solution. It is a quantitative method and the bacteria are concentrated on a membrane filter and then counted as colony forming unit (CFU). Principle of this method is based … Read more
What is Heat and Acetic Acid Test? Heat and acetic acid test (heat coagulation test) is a simple qualitative and semi quantitative test which is used for detection of excess protein in urine sample. Mainly albumin is detected but globulin may be present also. It is done as a screening test for proteinuria. Principle of … Read more
Sulfosalicylic acid (SSA) test is a simple screening test which is used to detect excessive proteins in urine. It is a rapid and low cost method and it is mainly used for checking proteinuria which indicates kidney impairment. The test is done on clear urine sample after centrifugation so that turbidity is observed clearly. It … Read more
What is Pauly’s Test? Pauly’s test is a biochemical colour test which is used for detection of tyrosine and histidine amino acid in proteins. It is a specific colourimetric assay. It was given by Hermann Pauly (German chemist) in 1904. It is based on azo-coupling reaction. In this process sulfanilic acid is diazotized in cold … Read more
PYR test (Pyrrolidonyl Arylamidase test) is a rapid colorimetric biochemical test used in clinical microbiology. It is done for detection of enzyme pyrrolidonyl arylamidase (pyrrolidonyl aminopeptidase) in bacteria. In this test the bacteria is exposed to a synthetic substrate L-pyrrolidonyl-β-naphthylamide. If the enzyme is present the substrate is hydrolyzed and β-naphthylamine is released which is … Read more
Urease test is a biochemical assay used in microbiology to detect whether microorganism produces urease enzyme or not. It is the process in which urea is broken down into ammonia and carbon dioxide by urease enzyme. When urease producing organism is inoculated in urease broth or agar the ammonia is rapidly accumulated and pH is … Read more
Nitrite Reduction Test is a biochemical test, which is used to determine that bacteria can reduce nitrite (NO2−) into other nitrogenous compounds. The nitrite is further reduced to nitric oxide (NO), nitrous oxide (N2O), nitrogen gas (N2) or ammonia (NH3). This test mainly indicates the ability of organism to remove nitrite from medium. In this … Read more
It is a rapid and simple test used for the determination of microbiological quality of milk. It is commonly employed in dairy laboratories to estimate the bacterial load present in milk samples. The test is based on the principle that actively growing bacteria utilize dissolved oxygen during their metabolic activities. Due to this oxygen consumption … Read more
Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.
The Butyrate Disk Test is a rapid biochemical test used in microbiology laboratory for the detection of enzyme butyrate esterase in bacteria. It is mainly used for the presumptive identification of Moraxella catarrhalis, which is an important human pathogen associated with respiratory tract infections. It is useful because this organism shows morphological similarity with Neisseria … Read more
