Nelson–Somogyi method is a classical colorimetric method used for the quantitative determination of reducing sugars such as glucose and fructose. It is a highly sensitive method and is commonly used in biochemical and carbohydrate analysis. It is considered as a standard method because the estimation is based on stoichiometric reduction reaction and gives accurate measurement … Read more
The Peroxide Value test is the process used to estimate the extent of primary oxidation in fats and oils. It is the measure of hydroperoxides formed at the initial stage of lipid oxidation, and it is expressed as milliequivalent of active oxygen per kilogram of oil. It is the major indicator of freshness because these … Read more
What is the Ninhydrin Test? It is the chemical test used for detecting amino acids and other compounds having free amino groups. It is the process in which ninhydrin (2,2-dihydroxyindane-1,3-dione) reacts with amino acids and the reaction is accompanied by oxidative deamination. In this step, the amino group is removed and there is release of … Read more
The phenylalanine deaminase test is a biochemical test used to identify bacteria that can deaminate the amino acid phenylalanine. It is the process where certain organisms convert phenylalanine into phenylpyruvic acid by removing the amine group. This is referred to as oxidative deamination and it is one of the important reactions for separating the PPM … Read more
The Bacitracin susceptibility test is one of the antimicrobial disc tests used to distinguish between group A and other -hemolytic Streptococci.
Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides.
The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci.
agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
Bial test is a biochemical test which is used for the detection of pentose sugars and to differentiate them from hexose sugars. It is mainly employed for identifying sugars like ribose and xylose and is also useful for the detection of RNA because RNA contains ribose sugar. This test is based on the reaction of … Read more
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