What is gas chromatography? Definition of Gas chromatography Gas chromatography (GC) is an analytical technique used to separate, identify, and quantify volatile compounds in a mixture by passing them through a column with a stationary phase and a carrier gas. Principle of Gas chromatography How does gas chromatography work? (Mechanism of Separation in Gas Chromatography) … Read more
Why we need to Validate an Autoclave? Validating an autoclave is crucial for ensuring the sterilization process is effective and reliable. Here’s why validation is necessary: Methods for Autoclave Validation Autoclave validation is a critical process in ensuring that sterilization procedures are effective and reliable. This is particularly important in the pharmaceutical industry, where sterility … Read more
When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.
What is Simple Microscope? Definition of Simple Microscope A simple microscope is an optical device consisting of a single convex lens that magnifies objects by enlarging their image through light refraction. Principle of Simple Microscope Working Mechanism of Simple Microscope This ray diagram in below, explains how simple microscopes is working; Magnification of a simple … Read more
An autoclave is a vital tool in scientific and medical laboratories, essential for ensuring the sterility of equipment and materials. Understanding its definition, principle of operation, key components, and operating procedures is crucial for students in these fields. This post delves into what an autoclave is, how it works, its main parts, and its diverse … Read more
By Sourav Pan 1 revisions 79 min readby Sourav Pan
What is pH Meter? A pH meter is a critical instrument used for measuring the acidity or alkalinity of aqueous solutions, quantified in terms of pH. The pH scale ranges from 0 to 14, where a value of 7 denotes neutrality, values below 7 indicate acidity, and values above 7 denote alkalinity. This scale reflects … Read more
What is HPLC (High-performance liquid chromatography)? Principle of HPLC (High-performance liquid chromatography) Instrumentation of HPLC High-Performance Liquid Chromatography (HPLC) is a sophisticated analytical technique that relies on a precise set of instruments to achieve effective separation, detection, and analysis of chemical compounds. The key components of an HPLC system include the mobile phase reservoir, solvent … Read more
Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.
What is Phenol-Sulfuric Acid Method for Total Carbohydrates? Objective of Phenol-Sulfuric Acid Method Principle of Phenol-Sulfuric Acid Method The principle of the Phenol-Sulfuric Acid Method centers on the reaction of carbohydrates with strong acid and heat, leading to the production of detectable compounds. This method involves the breakdown of carbohydrates—such as simple sugars, oligosaccharides, or … Read more
What is Osazone Test? The Osazone test is a biochemical method used to identify reducing sugars. This test is also known as the Phenylhydrazine test, referring to the reagent employed in the procedure. It distinguishes reducing sugars based on the time it takes for the osazone complex to form. Carbohydrates, or sugars, are defined chemically … Read more
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