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Northern Blotting – Protocol, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Western Blot – Protocol, Principle, Result.

Western Blot Protocol, Principle, Result

In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.

Giemsa Stain – Preparation, Procedure, Principle, Composition and Application

Giemsa Stain: Staining Procedure, Principle, Result and Application

The term Giemsa stain originated from a name of German chemist and bacteriologist Gustav Giemsa. He apply this stain with a combination of reagents to detect the presence of malaria parasites. This stain is used for nucleic acid staining and histopathological diagnosis of malaria and other parasites. Giemsa Stain is a types of Romanowsky stains … Read more

Nuclear Staining by Giemsa stain – Procedure, Principle, Result

Nuclear Staining by Giemsa stain: Procedure, Principle, Result

Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. Some … Read more

Cell Membrane Staining Procedure, Principle, Result

BACTERIAL CELL MEMBRANE STAINING

The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides the interior of the cell from the outside environment, thus protects the cell from environmental stress. The cell membrane is a lipid bilayer, contains cholesterols between phospholipids which maintain their fluidity at various temperatures. The membrane … Read more

Iron-Hematoxylin Staining – Principle, Procedure, Result

Iron-Hematoxylin Staining

Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).

Parasporal Crystal Stain – Principle, Procedure, Result

Parasporal Crystal Stain

Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.

Widal Test – Principle, Procedure, Result, Advantages, Limitation

Widal Test Principle, Procedure, Result, Advantages, Limitation

The Widal test is an agglutination test that determines any antibodies present in the patient’s serum that are generated against the causal agents of the enteric febrile illness (Salmonella Typhi, and Salmonella paratyphi B, A as well as C).

Counter Current Immunoelectrophoresis – Principle, Procedure, Result.

Counter Current Immunoelectrophoresis

Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells. 

Rocket Immunoelectrophoresis – Definition, Principle, Procedure, Result, Application

Rocket Immunoelectrophoresis Principle, Procedure, Result, Application.

Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample. 

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