Spread‌ ‌Plate‌ ‌Method‌ – Procedure, Principle, Purpose, Result.

Spread‌ ‌Plate‌ ‌Method‌: Procedure, Principle, Purpose, Result.

Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more

Pour Plate Method – Principle, Procedure, Objective, Result, Advantages

Pour Plate Method Principle, Procedure, Objective, Result

The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more

Streak Plate Method – Patterns, Procedure, Principle

Streak Plate Method: Patterns, Procedure, Principle

Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species.  During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more

Nitrate Reduction Test – Procedure, Principle, Result

Nitrate Reduction Test Procedure, Principle, Result

What is Nitrate Reduction Test? Purpose of Nitrate Reduction Test Principle of Nitrate Reduction Test Reactions of Nitrate Reduction Test Use of Zinc powder Use of  A Durham tube Requirements Composition of Nitrate broth (medium) Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Meat extract 3.000 Potassium nitrate 1.000 Sodium chloride 30.000 Final … Read more

Oxidase Test – Definition, Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Tollens’ Test Definition, Principle, Procedure, Result, Application.

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

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