IMViC test procedure, Purpose, Result, and Organism List.

Operating Procedure of Fluorescence spectroscopy

IMViC is a mnemonic that stands for four tests used in the identification of enteric bacteria: Indole, Methyl Red, Voges-Proskauer, and Citrate. These tests are commonly used in microbiology laboratories to identify bacterial species in the Enterobacteriaceae family, which includes many common pathogens such as Escherichia coli and Salmonella. The principle behind the IMViC tests … Read more

Alkaline Phosphatase Test of Milk – Determination of Phosphatase Activity of Milk

Alkaline Phosphatase Test of Milk- Determination of Phosphatase Activity of Milk

Alkaline Phosphatase (ALP) is an enzyme that is naturally found within all the raw milks that is utilized to determine the quality of pasteurization of milk. A complete pasteurization process can deactivate the enzyme below levels that can be detected by traditional methods. Because the stability of the heat of ALP is higher than the stability of pathogens that could be found in milk, it serves as a metric of security. However, failure for detection of ALP activity is not a mean an item is safe from pathogens.

Biochemical Test of Providencia stuartii

Biochemical Test of Providencia stuartii

Biochemical Test of Providencia stuartii Basic Characteristics Properties (Providencia stuartii) Capsule Non-Capsulated Catalase Positive (+ve) Citrate Positive (+ve) Flagella Flagellated Gas from glucose Negative (-ve) Gelatin Hydrolysis Negative (-ve) Gram Staining Negative (-ve) Growth in KCN Positive (+ve) H2S Negative (-ve) Indole Positive (+ve) Motility Positive (+ve) MR (Methyl Red) Positive (+ve) Nitrate Reduction Positive (+ve) … Read more

Oxidase Test – Definition, Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Tollens’ Test Definition, Principle, Procedure, Result, Application.

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

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