Pulsed-field Gel Electrophoresis (PFGE) – Definition, Types, Steps, Applications

Pulsed-field Gel Electrophoresis (PFGE) - Definition, Types, Steps, Applications

What is Pulsed-field Gel Electrophoresis (PFGE)? Principle of Pulsed Field Gel Electrophoresis (PFGE) The principle of pulsed-field gel electrophoresis (PFGE) revolves around the separation of DNA fragments based on their size using an electric field. In conventional gel electrophoresis, small DNA fragments migrate more easily through the gel matrix than large fragments. However, when dealing … Read more

PCR Machine – Definition, Principle, Parts, Types Steps, Applications, Examples

PCR Machine - Definition, Principle, Parts, Types Steps, Applications, Examples

What is PCR Machine? Principle of PCR The polymerase chain reaction (PCR) is based on a set of principles that enable the amplification of specific DNA sequences. The key components involved in PCR are the template DNA, DNA primers, DNA polymerase enzyme, and deoxynucleotide substrates. The principle of PCR begins with the selection of a … Read more

Electroporator – Definition, Principle, Types, Protocol, Applications

Electroporator – Definition, Principle, Types, Protocol, Applications

What is Electroporator (Electroporation machine)? What is Electroporation? Definition of Electroporator (Electroporation machine) An electroporator is a scientific instrument or device used in molecular biology and genetic engineering to facilitate the process of electroporation. Electroporation involves the application of electric pulses to cells in order to create temporary pores in their cell membranes, allowing the … Read more

Micropipettes – Parts, Types, Applications, Techniques

Micropipettes – Parts, Types, Applications, Techniques

What is a micropipette? What is the purpose of pipetting? Pipetting is the process of accurately measuring and dispensing specific volumes of liquid using a laboratory tool called a pipette. The main purpose of pipetting is to ensure precise and accurate manipulation of small volumes of liquid, which is essential in various laboratory applications such … Read more

Column Chromatography – Procedure, Instruments, Application, Advantages

Column Chromatography - Procedure, Instruments, Application, Advantages

What is Chromatography? What is Column Chromatography? Forms of Column Chromatography There are two forms of column chromatography. These different forms of column chromatography offer versatile options for separating and analyzing various types of compounds and mixtures. They find applications in diverse scientific fields, including pharmaceuticals, environmental analysis, forensic investigations, and biomolecular research. Principle of … Read more

Spectrophotometer – Principle, Parts, Types, Mechanism, Uses

Spectrophotometer - Principle, Parts, Types, Mechanism, Uses

What is a Spectrophotometer? Definition of Spectrophotometer A spectrophotometer is a laboratory instrument that measures the amount of light absorbed by a sample and is used to determine properties such as concentration or absorption patterns of substances. Principle of Spectrophotometer Spectrophotometry is a scientific technique that quantifies the intensity of light as a function of … Read more

Tissue Homogenizer – Definition, Parts, Types, Procedure

Tissue Homogenizer - Definition, Parts, Types, Procedure

A tissue homogenizer is a laboratory tool used to disrupt cells and tissues by mechanical means, such as grinding, blending, or shaking. It is commonly used in biology and biochemistry research to extract cellular components, such as proteins and nucleic acids, for further analysis. Homogenizers come in different types, including manual and mechanical versions, and … Read more

Ultrasonic Homogenizer – Principle, Parts, Types, Uses

Ultrasonic Homogenizer - Principle, Parts, Types, Uses

What is Ultrasonic Homogenizers? Principle of Ultrasonic Homogenizers The ultrasonic power supply converts line voltage to 20 kHz electrical energy at high frequency. This electrical energy is sent to the probe, which converts it into mechanical energy. The probe’s vibrations are connected to and amplified by the titanium tip. This longitudinal vibration is transmitted to … Read more

High Pressure Homogenizer – Principle, Types, Parts, Uses

High Pressure Homogenizer - Principle, Types, Parts, Uses

What is Homogenizer? What is High Pressure Homogenizer? Working principle of high pressure homogenizer High-pressure homogenization involves the use of high-pressure, compressed liquid material that flows through the gap at high speeds and is subjected to intense shear stresses. The collision of the liquid material on the metal ring generates a powerful impact force and … Read more

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