What is Flash chromatography? Principle of Flash column chromatography Flash column chromatography equipment Flash chromatography General consist of following parts 1. Pump Systems Type of pump There are different types of pumps commonly used in flash chromatography systems, each offering specific capabilities and functionality. Here are some notable types of pumps: The selection of a … Read more
What is Chromatography? Chromatography Definition Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description Gas … Read more
What is Two-Dimensional (Crossed) lmmunoelectrophoresis? Principle of Two-Dimensional lmmunoelectrophoresis The principle of two-dimensional (crossed) immunoelectrophoresis (2-D IEP) involves a two-step electrophoretic separation that permits antigen mixtures to be analysed and quantitated. In the first dimension the antigen (protein) mixture is loaded on an agarose gel and it is separated by electrophoresis by charge and mobility … Read more
What is Immunoelectrophoresis? Immunoelectrophoresis is defined as the separation of proteins by electrophoresis followed by their identification through immunologic (antigen-antibody) reactions. Immunoelectrophoresis Definition Immunoelectrophoresis is a biochemical technique that combines electrophoresis and antibody-antigen reactions to separate and identify proteins based on their antigenic properties. Objectives of Immunoelectrophoresis Principle of Immunoelectrophoresis The method is based on … Read more
What is Polyacrylamide Gel Electrophoresis (PAGE)? Definition of Polyacrylamide Gel Electrophoresis (PAGE) Polyacrylamide Gel Electrophoresis (PAGE) is a technique used to separate and analyze proteins or nucleic acids based on their mobility in a porous polyacrylamide gel matrix under the influence of an electric field. Types of Polyacrylamide Gel Electrophoresis (PAGE) Native PAGE – Proteins … Read more
What is SDS-PAGE? Definition of SDS-PAGE SDS-PAGE is a technique used to separate proteins based on their molecular weight by using a gel matrix and the denaturing agent sodium dodecyl sulfate (SDS). Objective Principle of SDS-PAGE SDS–PAGE refers as Sodium Dodecyl Sulfate–PolyAcrylamide Gel Electrophoresis, and its main principle based on separation of proteins mainly by … Read more
What is Microtome? Principle of Microtome Parts of a microtome Base – The base is used for giving stability and strong support to the whole instrument, it’s usually made of heavy metal so that vibration is reduced during cutting of tissue. Knife / Blade – The knife is employed for cutting the tissue sections into … Read more
What is Mass Spectrometry (MS)? Definition of Mass Spectrometry (MS) Mass Spectrometry (MS) is an analytical technique used to identify the chemical composition of a sample by measuring the mass-to-charge ratio of its ions, resulting in a mass spectrum that reveals the molecular and structural information of the sample. Principle of Mass Spectrometry (MS) Working of Mass … Read more
What is Solid State Fermentation (SSF)? Definition of solid state fermentation Solid State Fermentation (SSF) is a fermentation method that involves the growth of microorganisms on solid support, such as grain brans or oil seed cakes, without a free-flowing aqueous phase. It is used in various industries to produce valuable metabolites, including antibiotics, enzymes, and … Read more
What is IR Spectroscopy? Principle of IR Spectroscopy The basic principle is that molecules absorb infrared (IR) radiation when the frequency of the radiation matches a vibration of a chemical bond in the molecule. Vibrations in molecules (stretching, bending, twisting) are possible because atoms are held by bonds and the atoms move relative to each … Read more
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