Kirby Bauer Disk Diffusion Susceptibility Test Protocol

Kirby Bauer Disk Diffusion Susceptibility Test Protocol

What is Kirby Bauer Disk Diffusion test? The Kirby-Bauer disk diffusion test is a laboratory method used to determine the effectiveness of antibiotics against bacterial strains. The test is named after its inventors, Drs. Paul Kirby and Robert Bauer, who developed it in the 1960s. In the Kirby-Bauer test, a bacterial culture is spread evenly … Read more

Testing of Disinfectants (evaluation methods of disinfectants)

Testing of Disinfectants (evaluation methods of disinfectants)

Testing of Disinfectants/ Evaluation of disinfectants definition. Different methods for Testing of Disinfectants There are present different tests which can be used to test the efficiency of disinfectants such as; 1. Phenol coefficient test Methods of Phenol coefficient test There are two distinct methods of Phenol coefficient test, such as; A. Rideal Walker method for … Read more

Scope of Epidemiology

Scope of Epidemiology

Definition of Epidemiology Overview of Epidemiology Scope of Epidemiology/Objective of Epidemiology The objectives of epidemiology are to:  Major Areas of Discussion in Epidemiology The Major Areas of Discussion in Epidemiology are; Population Disease frequency Disease Distribution  Disease Determinants  Disease Control  Fields of Epidemiology

Measurement of Bacterial Growth

Measurement of Bacterial Growth

Measurement of Bacterial Growth Why Should We Measure Bacterial Growth? The following are reasons we measure bacterial growth: Methods used for Measurement of Bacterial Growth There are many methods available to measure bacterial growth. Each one is discussed below. Cell Number count The cell number counting is done by two following methods; A. Direct Method … Read more

Scope of Microbiology

Scope of Microbiology

Microbiology is among the most popular subjects after BiPC and is an important field of Biology that studies the study of fungi, bacteria virus, as well as other microorganisms that can’t be observed with eyesight. Microbiologists identify and study the behaviors of microbes under certain circumstances and the effect they may have on the environment. … Read more

RCF Centrifugation and RPM Centrifugation

RCF Centrifugation and RPM Centrifugation

When working with centrifuges you’ll encounter two different measurement units that are called the Relative Centrifugal Force (RCF) and the number of revolutions per Minute (RPM). It is essential to be aware of the fact that they are both different. If they are not used correctly (which isn’t as rare as you believe) and the … Read more

Sucrose Gradient Centrifugation

Sucrose Gradient Centrifugation

Sucrose gradient centrifugation Definition Technique for the characterization and preparation of subcellular particles. A sucrose gradient is used to fill a centrifuge tube of the type swinging-bucket design. The bottom is the most dense, and the top the least dense. The solution is then layered with a suspension of the particles. Centrifugation separates particles in … Read more

Centrifugation of blood – Blood Separation

Centrifugation of blood - Blood Separation

Centrifugation of blood  The process of dividing whole blood into its components is known as blood separation. For research purposes, diagnostic and therapeutic reasons as well as research, whole blood may be separated. Scientists may employ several blood separation methods to achieve this. Blood separation machines that spin at high speeds exert a centripetal force, … Read more

Filtration Sterilization – Types, Mechanism, Uses

Filtration Sterilization - Types, Mechanism, Uses

Filtration is the most popular method to sterilize heat sensitive liquid and gases, without exposure to denaturing temperatures. Instead of killing microorganisms that cause contamination the process simply eliminates them. It is the process of choice to sterilize chemical solutions for antibiotics radioisotopes, toxic chemicals or vaccines, as well as carbohydrates, all of which are … Read more

Radiation Sterilization – Types, Mechanism, Applications

Radiation Sterilization Types, Mechanism, Applications

Radiation is used to sterilize and decontaminate medical supplies (surgeon supplies, vaccines and drugs) as well as the food and beverage industries. There are two types of radiations available: ionizing or non-ionizing. Radiation sterilization isn’t widely used in the food industry because of concerns about radioactive contamination, toxic or carcinogenic products production, taste alteration, and … Read more

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