Types of PCR with Definition, Principle, and Uses

Types of PCR with Definition, Principle, and Uses

Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR Conventional PCR is the basic type of PCR in which target DNA is amplified by repeated temperature cycles. It is also called end-point PCR or traditional PCR. In this method, detection of amplified DNA is done only … Read more

PCR Machine – Principle, Parts, Types Steps, Applications

PCR Machine - Definition, Principle, Parts, Types Steps, Applications, Examples

PCR Machine is an automated laboratory instrument that is used to amplify small segment of DNA or RNA into millions or billions of copies. It is also known as thermal cycler or DNA amplifier. It contains a thermal block where reaction tubes containing sample, primers, nucleotides and polymerase enzyme are placed. The machine changes the … Read more

Electroporator – Principle, Types, Protocol, Applications

Electroporator – Definition, Principle, Types, Protocol, Applications

Electroporator is a specialized electronic pulse generator that is used to deliver controlled high-voltage electrical pulses to biological cells. It is used in electroporation process for temporary opening of the cell membrane. It acts on the lipid bilayer membrane of the cell and makes small aqueous pores for short time. Through these pores, different macromolecules … Read more

Micropipettes – Parts, Types, Applications, Techniques

Micropipettes – Parts, Types, Applications, Techniques

Micropipette is a laboratory instrument that is used to measure and transfer very small volume of liquid in microliter range. It is highly accurate instrument and commonly used in molecular biology, chemistry and clinical diagnostic laboratory. It works by a piston mechanism which creates suction and draws liquid into a disposable plastic tip. The volume … Read more

Column Chromatography – Principle, Steps, Parts, Uses, Advantages

Column Chromatography - Procedure, Instruments, Application, Advantages

Chromatography is a laboratory analytical technique which is used to separate a chemical mixture into its individual components. It is used for identification, purification and estimation of different substances present in a mixture. It separates the components according to their different movement through two phases. In this technique, the mixture is distributed between a mobile … Read more

Spectrophotometer – Principle, Parts, Types, Mechanism, Uses

Spectrophotometer - Principle, Parts, Types, Mechanism, Uses

Spectrophotometer is an analytical instrument which is used to measure the light absorbed, transmitted or reflected by a substance at particular wavelength. It has a light source, sample holder and detector. The liquid sample is kept in a cuvette. Then the beam of light is passed through the sample. The detector detects the light which … Read more

Single Use Bioreactor – Principle, Parts, Types, Uses

Single Use Bioreactor - Principle, Parts, Types, Uses

Single Use Bioreactor is a cell cultivation system where the reusable glass or stainless steel vessel is replaced by a disposable plastic bag. It is also known as Disposable Bioreactor. The bag is pre-sterilized and used only for one batch. This flexible polymeric bag acts as the sterile place for the growth of cells. It … Read more

Tissue Homogenizer – Principle, Parts, Types, Procedure

Tissue Homogenizer - Definition, Parts, Types, Procedure

Tissue Homogenizer is a laboratory device that is used to break biological tissues into small and uniform particles. It physically disrupts the tissue structure by rupturing the cell wall and cell membrane. During this process, intracellular components like proteins, nucleic acids (DNA and RNA) and microorganisms are released from the tissue matrix. The uniform mixture … Read more

Ultrasonic Homogenizer – Principle, Parts, Types, Uses

Ultrasonic Homogenizer - Principle, Parts, Types, Uses

Ultrasonic Homogenizer is a scientific device used to disrupt, mix and homogenize liquid materials. It is also called sonicator or ultrasonic processor. It is used to make uniform dispersion and emulsion in liquid sample. It mainly consists of three parts, such as generator, piezoelectric transducer and metal probe. The metal probe is also called horn … Read more

High Pressure Homogenizer – Principle, Types, Parts, Uses

High Pressure Homogenizer - Principle, Types, Parts, Uses

Homogenizer is a mechanical device used to make a uniform and stable mixture of two substances which normally do not mix well. It is used in food, dairy, pharmaceutical and cosmetic industries. It forces a liquid or semi-fluid product through a narrow gap called homogenizing valve under very high pressure. In this process, the liquid … Read more