Sourav Pan
The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.
OF (Oxidation-Fermentation) test is a biochemical test used to find out how a bacterium utilize carbohydrate (mainly glucose). It was developed by Hugh and Leifson (1953). It differentiates organisms into fermentative type and oxidative type based on acid production in anaerobic and aerobic condition. It is done in a semi-solid OF medium having high carbohydrate … Read more
Pyruvate Broth Test is a biochemical test used to determine the ability of a bacterium to utilize pyruvate as a carbon and energy source. It is done in a carbohydrate-free broth medium containing sodium pyruvate. The medium contains an indicator (bromothymol blue) which gives greenish-blue colour initially. If the organism metabolizes pyruvate, organic acids are … Read more
Phenylalanine Deaminase Test (PDA test) is a biochemical test used to detect the enzyme phenylalanine deaminase produced by some bacteria. It is used to identify and differentiate gram-negative enteric bacteria, mainly the Proteeae group from other Enterobacteriaceae. In this test, the organism is grown on a medium containing phenylalanine. If the enzyme is present, phenylalanine … Read more
The Bacitracin susceptibility test is one of the antimicrobial disc tests used to distinguish between group A and other -hemolytic Streptococci.
Streptococcus pneumoniae can be distinguished from other viridans streptococci by showing that it is sensitive to optochin, dissolves in bile, reacts with a specific DNA probe, or has species-specific capsular polysaccharides.
The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci.
agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
Bial’s test is a chemical test used for detection of pentoses (5-carbon sugars) and it is used to distinguish pentoses from hexoses. It was developed by German physician Manfred Bial. It is also used in laboratory for estimation of RNA because RNA contains pentose sugar ribose. It works on principle of acid dehydration of sugars. … Read more
