Bombardment (Biolistics) Method for Plant Cells – Principle, Requirement, Protocol

Bombardment (Biolistics) Method for Plant Cells - Principle, Requirement, Protocol

What is Bombardment (Biolistics) Method? Bombardment (Biolistics) method is a direct physical technique used to introduce foreign DNA into living cells. It is also referred to as the gene gun method. In this process, the desired DNA is first coated on very small and dense metal particles such as gold or tungsten. These DNA coated … Read more

Electrofusion Technique for Plants – Requirement, Protocol, Advantages, Uses

Electrofusion between a celery cell suspension protoplast and a lovage meso- phyll protoplast.

What is the Electrofusion of Plant Cells? Electrofusion is a technique used for the fusion of two or more plant cells by the application of an electric field. It is commonly used for the fusion of plant protoplasts in plant biotechnology. In this method the plasma membranes of adjacent cells are temporarily disturbed and the … Read more

Classification of Fungi – Gymnomycota, Mastigomycota, Amastigomycota.

Classification of Fungi

The fungi are classified based on the characteristics of the sexual spores and fruiting bodies present during the sexual stages of their life cycles. Therefore, based on characteristics of their sexual stages and morphology of their asexual spores and thalli they are classified into different groups.

Cambridge O Level Biology Study Notes

Cambridge O Level Biology Study Notes

Cambridge O Level Biology is an internationally recognised school-level biology course and exam offered by Cambridge International for students usually aged 14 to 16. It helps students understand the basic concepts of biology, such as cells, plants and animals, human body systems, genetics, and the environment. The course also teaches practical skills through experiments and … Read more

AP Biology Study Notes  – Get free AP Biology Study Notes

AP Biology Study Notes

The AP Biology exam is a college-level biology test that is conducted by the College Board, and it is usually taken by high-school students who want to show that they have strong understanding in biology. It is the process where different parts of biology like molecules and cells, organisms, and ecosystems are studied, and the … Read more

Methylene Blue Reduction Test (MBRT) – Principle, Procedure, Result

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It is a rapid and simple test used for the determination of microbiological quality of milk. It is commonly employed in dairy laboratories to estimate the bacterial load present in milk samples. The test is based on the principle that actively growing bacteria utilize dissolved oxygen during their metabolic activities. Due to this oxygen consumption … Read more

Cetrimide Agar Test – Principle, Purpose, Procedure, Results

Cetrimide Test - Principle, Purpose, Procedure, Results

Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.

Butyrate Disk Test – Principle, Procedure, Result, Uses

Butyrate Disk Test Principle, Procedure, Result

The Butyrate Disk Test is a rapid biochemical test used in microbiology laboratory for the detection of enzyme butyrate esterase in bacteria. It is mainly used for the presumptive identification of Moraxella catarrhalis, which is an important human pathogen associated with respiratory tract infections. It is useful because this organism shows morphological similarity with Neisseria … Read more

DNase Test – Principle, Procedure, Result, Uses

Deoxyribonuclease (DNase) Test Principle, Procedure, Result

The DNase test is a differential biochemical test which is used to detect the ability of microorganism to produce an extracellular enzyme called deoxyribonuclease (DNase). It is the process where polymerized DNA present in the medium is hydrolyzed into smaller nucleotide fragments by the action of this enzyme. These degraded products can be absorbed by … Read more

Acetamide utilization Test – Principle, Procedure, Result, Uses

Acetamide utilization Test Principle, Procedure, result

It is a biochemical test used for the identification and differentiation of non-fermentative Gram-negative bacteria. It is mainly applied for the confirmation of Pseudomonas aeruginosa. This test is based on the ability of the organism to utilize acetamide as the sole source of carbon and nitrogen. The test medium contains acetamide and a pH indicator … Read more