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Nitrocefin Test (Beta-Lactamase Test) – Principle, Procedure, Uses

Nitrocefin test - Principle, Procedure, Uses

The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.

Mucic acid test – Principle, Procedure, Result, Uses

Mucic acid test - Principle, Procedure, Result

Mucic acid test is a specific chemical test used for the identification of galactose and galactose containing sugars like lactose. It is also referred to as galactaric acid test. It is based on the oxidation reaction of sugars with concentrated nitric acid. In this test the sugar sample is heated with concentrated nitric acid which … Read more

Molisch Test – Objective, Principle, Procedure, Result

Molisch’s Test Objective, Principle, Procedure, Result

This is a test that is common for all carbohydrate larger than the tetroses. The test works on the basis that pentoses as well as hexoses can be dehydrated using conc. sulfuric acid, resulting in furfural or hydroxyl-methyl furfural or hydroxyl methyl furfural. These compounds condense with a-naphthol to create a purple condensation product. Molisch’s … Read more

Rapid Furfural Test for Glucose and Fructose – Principle, Procedure, Result

Rapid Furfural Test for Glucose and Fructose

Rapid Furfural Test is a qualitative chemical test used to differentiate ketohexoses from aldohexoses based on the rate of dehydration reaction. It is mainly used for the identification of fructose and other ketose sugars in the presence of aldose sugars like glucose. It is based on the principle that ketohexoses are dehydrated rapidly when heated … Read more

Fehling’s Test – Principle, Procedure, Result

Fehling's Test Principle, Procedure, Result

This test also can differentiate between ketone functional groups and water-soluble carbohydrates.

Solubility Tests of Proteins – Principle, Procedure, Result, Uses

Solubility Tests of Proteins - Principle, Procedure, Result, Application

Solubility Tests is a type of biochemical test which is performed to check the ability of compounds to dissolve within a liquid solvent.

Alkaline Phosphatase Test of Milk – Principle, Procedure, Result, Uses

Alkaline Phosphatase Test of Milk- Determination of Phosphatase Activity of Milk

Alkaline Phosphatase (ALP) is an enzyme that is naturally found within all the raw milks that is utilized to determine the quality of pasteurization of milk. A complete pasteurization process can deactivate the enzyme below levels that can be detected by traditional methods. Because the stability of the heat of ALP is higher than the stability of pathogens that could be found in milk, it serves as a metric of security. However, failure for detection of ALP activity is not a mean an item is safe from pathogens.

Biochemical Test of Providencia stuartii

Biochemical Test of Providencia stuartii

The biochemical test of Providencia stuartii refers to the group of laboratory tests that are used to identify this bacterium on the basis of its metabolic and enzymatic activities. It is a Gram-negative rod shaped facultative anaerobic organism belonging to the family Morganellaceae. In clinical microbiology these tests is carried out to differentiate Providencia stuartii … Read more

Carbohydrate Fermentation Test – Sugar Fermentation Test

Carbohydrate Fermentation Test - Sugar Fermentation Test

The carbohydrate fermentation test can be used to determine if bacteria are able to ferment a certain carbohydrate.
It is a test to determine any presence of acids or gas resulting from carbohydrate fermentation.

IMViC Test – Principle, Procedure, Result, Uses, Examples

Operating Procedure of Fluorescence spectroscopy

What is IMViC test? The IMViC test is a group of biochemical tests which is used for the identification and differentiation of bacteria belonging to the family Enterobacteriaceae. It is mainly used to distinguish fecal coliform bacteria from non-fecal coliform bacteria. The term IMViC is an acronym which stands for Indole, Methyl Red, Voges–Proskauer and … Read more

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