Sourav Pan
What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more
What is Repetitive sequence-based PCR (rep-PCR)? Working Principle of rep-PCR The rep-PCR working principle is based on the occurrence of conserved repetitive DNA elements (REP, ERIC, BOX, GTG5 etc.), which are dispersed at multiple sites within prokaryotic genomes, and these elements serve as priming sites for amplification. By the use of specifically designed primers, annealing … Read more
What is Nanoparticle-Assisted PCR (nanoPCR)? Working Principle of Nanoparticle-Assisted PCR (nanoPCR) The nanoPCR method is introduced with nanoparticles (NPs) being added into a conventional PCR mixture which contains template DNA, primers, DNA polymerase, dNTPs, and buffer. With optimal NP size (often <100 nm) and concentration being selected, thermal conductivity is enhanced by NPs which accelerates … Read more
Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR 2. High-fidelity PCR 3. Hot-Start PCR 4. Fast-cycling PCR 5. Touch down (TD) PCR 6. Real-Time PCR (qPCR) 7. Digital PCR (dPCR) 8. High-Resolution Melt (HRM) PCR 9. Reverse-Transcriptase PCR (RT-PCR) 10. RT-qPCR – Real-time quantitative reverse transcription … Read more
Microbiology in nursing is the scientific study of microscopic organisms such as bacteria, virus, fungi and protozoa that impact human health in clinical and community care. it deals with how these microbes grow, spread, and survive, also how nurses can prevent or control infections. this knowledge is used daily in patient care, wound dressing, vaccination … Read more
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
What is Brucella? Scientific classification of Brucella Domain: Bacteria Phylum: Pseudomonadota Class: Alphaproteobacteria Order: Hyphomicrobiales Family: Brucellaceae Genus: BrucellaMeyer and Shaw 1920 (Approved Lists 1980) Characteristics of Brucella Geographical Distribution of Brucellosis Habitat of Brucella Classification of Brucella Species: The genus Brucella comprises both classical and novel species, with some formerly classified under the genus … Read more
Staphylococcus aureus is a Gram‑positive, facultative anaerobic bacterium, usually found in clusters that resemble grapes and typically 0.5–1µm in diameter. Harbor, as a commensal organism, in the human nasal passages, skin, and mucous membranes of 20–30% of people without causing disease It is an opportunistic pathogen that can cause a wide range of infections—from simple … Read more
Pectin is a complicated polysaccharide that is mostly found in the cell walls of plants, notably fruits. It is a structural heteropolysaccharide made up mostly of units of galacturonic acid. Pectin is a glue that holds plant cells together, making the plant tissue strong and stable. In the culinary sector, it is often used as … Read more
A biosafety level (BSL) refers to a specific set of biocontainment measures designed to isolate hazardous biological agents within a controlled laboratory environment. This document outlines the necessary protective measures required in a laboratory environment to safeguard employees, the ecosystem, and the community from infectious agents. Historical Overview The classification of four levels of biosafety … Read more
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