Tollens’ Test – Principle, Procedure, Result, Application

Tollens’ Test Definition, Principle, Procedure, Result, Application.

It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.

Oxidase Test – Principle, Procedure, Result, Application

Oxidase Test Definition, Principle, Procedure, Result, Application

The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .

Gerhardt’s test – Principle, Procedure, Result

Gerhardt's test

Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.

Nitrate Reduction Test – Procedure, Principle, Result

Nitrate Reduction Test Procedure, Principle, Result

What is Nitrate Reduction Test? Nitrate reduction test is a biochemical test used to determine the ability of bacteria to reduce nitrate (NO₃⁻) into nitrite (NO₂⁻) or into other nitrogenous compounds such as ammonia or nitrogen gas. It is the process in which nitrate acts as a terminal electron acceptor during anaerobic respiration. This test … Read more

Simmons Citrate Agar – Principle, Composition, Procedure, Result, uses

Simmons citrate agar test

Simmons Citrate Agar is a chemically defined medium in which sodium citrate act as the only carbon source and ammonium dihydrogen phosphate act as the only nitrogen source. It is the medium developed as a modification of Koser’s citrate medium and it is used mainly to differentiate members of Enterobacteriaceae. It is the process in … Read more

Reverse CAMP test – Principle, Procedure, Result, Uses

Reverse CAMP test

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Northern Blotting – Steps, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Cell Membrane Staining – Principle, Procedure, Result, Uses

Cell Membrane Staining

Cell membrane staining is the process in which the plasma membrane of a cell is visualized with the help of dyes or fluorescent probes. It is the thin outer covering of the cell, and it is normally transparent, so it cannot be seen clearly under a simple light microscope. It is the process where special … Read more

Giemsa Stain- Principle, Procedure, Results, Uses

Procedure of Giemsa staining

Giemsa stain is a polychromatic nucleic acid stain that is used mainly for demonstrating different blood cells and parasites in stained blood films. It is named after the German chemist Gustav Giemsa. It belongs to a group of stains known as Romanowsky stains. These are neutral stains made from a mixture of oxidized methylene blue, … Read more

Capsule Staining – Procedure, Principle, Result

Maneval's capsule staining

Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.