Southern Blot – Definition, Principle, Steps, Importance
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
Protocols
In this category, you will find protocols for different biological experiments.
Plant Tissue Culture – Definition, techniques, Media composition, types, application.
What is Plant Tissue Culture? Plant tissue culture Definition Plant tissue culture is a biotechnology technique that involves growing plant cells, tissues, or organs in a controlled, sterile environment on a nutrient medium. This method exploits the totipotency of plant cells, allowing for the rapid propagation of plants, production of disease-free specimens, and conservation of … Read more
Northern Blotting – Protocol, Principle, Application, Result
Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.
Western Blot – Protocol, Principle, Result.
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
Polymerase Chain Reaction (PCR) – Definition, Steps, Principle, Application
Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).
Blood Grouping Test – Principle, Procedure, Result
The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.
Gel Staining Procedure for PAGE
Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.
Laboratory Rules – Basic Rules Of A Microbiology Laboratory
A well-established microbiology laboratory is necessary for the work of microorganisms. The students/researchers/scientists must follow the basic rules while working with microorganisms.
Measurement Of Microorganisms – Micrometry
A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.
Giolitti-Cantoni Broth for Staphylococcus aureus
Giolitti Cantoni Broth Base with the addition of potassium tellurite is utilized to enrich selectively Staphylococcus aureus, a food-borne pathogen, from suspected items, according to ISO.