The novobiocin (antibiotic) differentiation disc can be used in the Novobiocin Susceptibility Test to help make a preliminary distinction between Staphylococcus saprophyticus and other coagulase-negative staphylococci (CoNS) in clinical (urinary) specimens.
The Oxidation–Fermentation (OF) test is a biochemical test that is used to differentiate bacteria on the basis of their ability to metabolize carbohydrates by oxidative or fermentative pathways. It is the process that helps in identifying whether the organism utilizes carbohydrate in the presence of oxygen or in the absence of oxygen. This test was … Read more
Pyruvate Broth Test is a biochemical test that is used in microbiology for identification and differentiation of certain bacteria. It is mainly used for differentiating species of Enterococcus group. It is the process in which ability of organism to utilize sodium pyruvate as a sole source of energy is determined. The medium used in this … Read more
The o-Nitrophenyl-β-D-Galactopyranoside (ONPG) test is a biochemical test used for the detection of the enzyme β-galactosidase in bacteria. It is mainly applied for differentiating late lactose fermenting organisms from non-lactose fermenters. It is based on the ability of the organism to hydrolyse a synthetic lactose analogue when the required enzyme is present. The test is … Read more
The ability to develop in the presence of varying concentrations of sodium chloride (NaCl) has been used to distinguish between various bacteria.
The Sulfur Reduction Test is the biochemical procedure that is used to detect whether a microorganism is capable of reducing the sulfur-containing compounds to produce hydrogen sulfide (H₂S). It is the process which mainly helps in identifying members of Enterobacteriaceae where some bacteria like Salmonella, Proteus and Citrobacter are able to form the gas during … Read more
Growth at 42°C test is a physiological test used in microbiology for the identification and differentiation of certain non-fermentative Gram-negative bacteria. It is the process where the organism is tested for its ability to grow and survive at a higher temperature of 42°C. This test is mainly applied for the differentiation of Pseudomonas aeruginosa from … Read more
The MRS formulation was made by de Man, Rogosa, and Sharpe to replace a variable product (tomato juice) and give lactobacilli in general a good place to grow.
Lysine decarboxylase test relies on the capability of certain bacteria to degrade L-lysine into caverine after the release of carbon dioxide.
The starch hydrolysis test is a biochemical test which is used to determine the ability of microorganism to hydrolyze starch. It is also referred to as amylase test. It is the process in which extracellular enzymes are produced by the bacteria to break down starch into simpler sugars. These enzymes include α-amylase and oligo-1,6-glucosidase. Starch … Read more
