Aim Transfer of microbial cells from a parent culture medium to a fresh broth culture. Principle The broth is a liquid medium, which is used to grown microorganisms within the laboratory on a small scale. It also used in an industrial fermentor on a large scale. The media called broths, milks, or nutrient solutions are … Read more
Streak Plate Method Overview There are two types of microbial culture such as mixed culture and pure culture. The mixed culture contains two or more species while the pure culture contains only a single species. During the identification of a microorganism, the first and important step is to isolate the individual species from a mixed … Read more
Glucose, a key sugar molecule, plays a vital role in the human body as the primary carbohydrate found in the bloodstream. Its importance lies in its function as the main source of energy for various body tissues, notably the brain. When glucose is metabolized, it undergoes a sequence of chemical processes that result in the … Read more
What is Folin-Wu Method? Principle of Folin-Wu Method Requirements Procedure of Folin-Wu Method Step 1: Preparation of Protein-Free Filtrate Step 2: Testing Procedure This process results in a color change proportional to the glucose concentration in the blood sample. By comparing the absorbance of the test sample against the standard, the glucose level in the … Read more
What is Lane-Eynon Method? Principle of Lane-Eynon Method The Lane-Eynon method is a titrimetric procedure that quantifies reducing sugars in a solution through the utilization of Fehling’s solution as the primary reagent. This method operates on the fundamental chemistry of reduction-oxidation reactions, where Fehling’s solution, composed of copper sulfate and an alkaline solution of sodium … Read more
Milk is widely recognized as a significant source of calcium, an essential mineral that plays a crucial role in the development and maintenance of strong bones and teeth. This mineral is particularly important for infants and young children, whose rapidly growing bodies demand high levels of calcium to ensure proper bone formation and density. Calcium’s … Read more
Magnesium is an essential mineral found in many foods, including milk. It plays a vital role in numerous bodily functions, such as muscle and nerve function, blood sugar control, and bone health. The magnesium content in milk contributes to its nutritional value, making milk a beneficial part of a balanced diet. The amount of magnesium … Read more
Phosphorus is an essential mineral found in many foods, including milk. It plays a critical role in various bodily functions such as the formation of bones and teeth, energy production, and the synthesis of RNA and DNA, which are crucial for the growth and repair of cells. In milk, phosphorus is naturally present and contributes … Read more
What is the Litmus Milk Test? Litmus Milk Medium is a liquid medium suggested for use in qualitative tests for determining the activity of bacteria on milk and maintaining lactic acid bacteria. Acid Reaction Alkaline Reaction Redox Reaction Principle of Litmus Milk Test Purpose of Litmus Milk Test Media Required This test utilises Litmus Milk … Read more
What is Nitrate Reduction Test? Purpose of Nitrate Reduction Test Principle of Nitrate Reduction Test Reactions of Nitrate Reduction Test Use of Zinc powder Use of A Durham tube Requirements Composition of Nitrate broth (medium) Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Meat extract 3.000 Potassium nitrate 1.000 Sodium chloride 30.000 Final … Read more
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