Practical Archives - Page 12 of 53 - Biology Notes Online

Culture Media – Definition, Types, Composition, Use, Examples

• Published on January 21, 2025 • 31 min readby Editorial Team

When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.

Benedict’s Test – Principle, Reagents, Procedure, Result, Limitation

• Published on January 20, 2025 • 17 min readby Editorial Team

Benedict’s Test - Principle, Reagent Preparation, Procedure, Result, Limitation

Benedict’s test is a chemical test that is used to test for the presence of reduced sugars within an analytical test. Thus, simple carbohydrates that contain an aldehyde or free ketone functional group are detected using this test. The test is basing itself upon Benedict’s Reagent (also called Benedict’s solution) which is a complex mix of sodium carbonate, sodium citrate, and the pentahydrate of copper(II) Sulfate.

Electron Microscope – Principle, Types, Parts, Application, Diagram

• Published on January 16, 2025 • 34 min readby Editorial Team

An electron microscope is an instrument which produces an image of a sample by means of a beam of electrons. Optical microscopes use light to form an image, electron microscopes have greater resolution, and can be used to observe objects that are too small to be seen with an optical microscope. In a large variety … Read more

Baran Pipette Column – Parts, Procedure, Types, Uses

• Published on January 15, 2025 • 14 min readby Sourav Pan

What is Baran Pipette Column? Principle of Baran Pipette Column Principles of adsorption chromatography are used in the Baran pipette column. In this technique, the separation of compounds is achieved through their differential interactions with two key phases: in the stationary phase and the mobile phase. The pasteur pipette is packed with a stationary phase, … Read more

Gas Chromatography – Definition, Parts, Principle, Working, uses

• Published on January 13, 2025 • 40 min readby Sourav Pan

What is gas chromatography? Gas chromatography (GC) is a sophisticated technique that separates, identifies, and quantifies chemical components in complex mixtures. It operates on the principle of distributing the components between a mobile phase and a stationary phase. In GC, the mobile phase is an inert gas like helium, and it acts as a carrier … Read more

Mohr Pipette – Parts, Types, Procedure, Uses

• Published on December 5, 2024 • 10 min readby Sourav Pan

What is Mohr Pipette (graduated pipette)? Parts of Mohr Pipette (graduated pipette) A Mohr pipette, widely used in laboratories for liquid measurement, consists of several components designed to enable accurate and efficient operation. Below are the main parts and their functions: How to Use a Mohr Pipette (graduated pipette) A Mohr pipette is commonly used … Read more

Pipette Dropper – Parts, Types, Procedure, Uses

• Published on December 3, 2024 • 16 min readby Sourav Pan

What is Pipette Dropper? Components of Dropper Pipettes A dropper pipette consists of several key components, each serving a specific purpose in the process of transferring small amounts of liquid. Understanding the function of these parts is crucial for ensuring accurate and efficient liquid dispensing. Types of Dropper Pipettes Dropper pipettes come in several variations, … Read more

Repeater Pipette – Parts, Procedure, uses

• Published on December 3, 2024 • 8 min readby Sourav Pan

What is Repeater Pipette? How Repeater Pipettes Work Repeater pipettes enable the precise and repetitive dispensing of liquid in equal volumes. They improve efficiency by eliminating the need for repeated aspiration. Here’s a breakdown of their operation: Mechanism of Action Parts of Repeater Pipettes Repeater pipettes are designed for precise and efficient liquid dispensing. They … Read more

Mouth Pipette – Parts, Procedure, Uses, Drawbacks

• Published on December 3, 2024 • 11 min readby Sourav Pan

What is mouth pipette? Principle of Mouth Pipette The working principle of mouth pipetting is based on the creation of a pressure differential, which allows for the movement of liquid into a pipette through suction. This method involves the use of a glass capillary tube, where one end is submerged in the liquid to be … Read more

Multichannel Pipette – Parts, Types, Procedure, Applications

• Published on December 3, 2024 • 14 min readby Sourav Pan

What is Multichannel Pipette? Key Features of Multichannel Pipettes Multichannel pipettes are invaluable in laboratory settings, allowing users to transfer liquids across multiple channels simultaneously. Their design and functionality provide significant advantages for a range of tasks, particularly when handling large numbers of samples. Below are the core features that make multichannel pipettes essential tools … Read more