Hanging Drop Method (Motility test) – Principle, and Procedure, Result, Uses
Hanging drop technique allows for the analysis of living microorganisms. This involves fixing the Microbial suspension into a drop of liquid over the slide of glass.
Practical
Subcategories
Bacterial Smear – Principle, Preparation Steps, Uses, Limitations
A bacterial smear is a thin layer of bacterial cell/culture which is placed on a clean, grease-free microscopic slide to observe a clear image under a microscope.
Native Polyacrylamide Gel Electrophoresis (PAGE)
Native PAGE is the method where proteins are separated in their original folded condition without any use of denaturing reagents. It is the process in which the movement of proteins in a polyacrylamide gel is controlled by the natural charge, size and the native conformation of the molecules. The proteins migrate through the gel matrix … Read more
Bioreactor – Types, Design, Parts, Applications, Limitations
A bioreactor is the vessel in which biological reactions is carried out in controlled conditions. It is the process where microorganisms, plant cells or animal cells are allowed to grow and produce useful products. It is designed as a closed container in which temperature, pH, dissolved oxygen (DO) and nutrient level is maintained with the … Read more
Microtome – Principle, Parts, Types, and Uses
What is Microtome? A microtome is a laboratory device that is used for cutting very thin sections of different materials for microscopic examination. It is the instrument by which uniform slices are produced, and it is the process that helps the light or the electron to pass through the specimen so that the internal structures … Read more
Latex agglutination test – Definition, Procedure, Principle, Advantages, Limitation, Uses
Latex agglutination test is a simple serological test where antigen and antibody reaction is made visible on the surface of latex particles. It is the process in which latex beads are coated either with antigen or with antibody and when a sample containing the corresponding component is added, the beads is clumped together forming visible … Read more
Spectrophotometer Cuvette – Features, Types, Materials, Precautions, Advantages
A spectrophotometer cuvette is a small optical cell that is used to hold the liquid sample during absorbance or transmittance measurement. It is usually made in a rectangular shape and two opposite sides are kept clear and highly polished so that the light beam can pass through the solution without scattering. It is the process … Read more
Temporary Wet Mount technique – Procedure, Requirement, Uses
In Mounting technique, the specimen to be observed is held on a microscopic slide by using a coverslip.
How to Calibrate ph Meter? Step By step procedure and requirement
A pH meter is an instrument that is used to measure the pH of a solution, and it is the value that shows how acidic or alkaline the solution is. It is the process where a glass electrode and a reference electrode are placed in the sample, and the potential difference formed between them is … Read more
Nylander’s Test for Carbohydrates Principle, Procedure, Result
Nylander’s test is a qualitative test that is used for detecting reducing sugars. It is the process where the reducing sugar is heated with an alkaline solution containing bismuth salt, and the reaction is controlled by the components present in the reagent. The reagent is made of bismuth nitrate or bismuth subnitrate, potassium sodium tartrate, … Read more