What is Oil Immersion? What is Microscopic Resolution? Microscopic Resolution Definition The microscopic resolution also is known as resolving power. Resolution is defined as the ability of a lens to separate or distinguish between small objects that are close together or the ability to distinguish two adjacent points as distinct and separate. Equation of Microscopic … Read more
Differential centrifugation (also called the differential velocity method) is a common process in the fields of biochemistry and cell biology it is utilized to differentiate organelles and other subcellular particles by their sedimentation rates. Although it is commonly used in biochemical analysis it is a general method that is useful for the crude elimination of … Read more
Autoclaving is one method that uses steam to sterilize. Autoclave bags are utilized for sterilization at high temperatures to keep low-temperature plastics in the autoclave bags from sticking against the side of the sterilizer or obstructing the plumbing for the sterilizer’s vent. Autoclaves function by using a combination of pressure, steam, and time. They work … Read more
Density Gradient Centrifugation Density gradient centrifugation refers to an approach to separation between molecules, where the separation is determined by the density of the molecules when they travel through a gradient under the force of centrifugal. Principle of Density gradient centrifugation Density gradient centrifugation relies on the premise that molecules settle under the force of … Read more
What is Laminar flow hood/cabinet? Definition of Laminar flow Laminar flow refers to the smooth, uninterrupted flow of fluid, such as air or liquid, in parallel layers without any significant mixing or turbulence. Principle of Laminar flow hood The working principle of a laminar flow hood is based on creating a controlled airflow within the … Read more
What is transmission electron microscope? Parts of A Transmission Electron Microscope 1. Electron Gun Types of Electron Gun Used in Transmission Electron Microscope There are several types of electron guns that are used in transmission electron microscopes (TEMs), including: Overall, the type of electron gun used in a TEM depends on the specific requirements and … Read more
A scanning electron microscope (SEM) is a type of electron microscope that generates images by scanning the surface of a specimen with a concentrated beam of electrons. Electrons interact with atoms in the sample, generating signals that carry information about the sample’s surface topography and composition. The electron beam is scanned in a raster scan … Read more
What is phase contrast microscopy? A phase contrast microscopy converts slight differences in refractive index and cell density into easily detected variation in light intensity to observe living cells. This microscope is used for visualization of cell culture and live cells. Living cells can be observed without any staining. Unstained specimens have absorbed no light, … Read more
Confocal microscopy has various advantages over conventional optical microscopy, including a short depth of field, the elimination of out-of-focus glare, and the ability to collect optical slices serially from thick specimens. A prominent use of confocal microscopy in the biomedical sciences is the imaging of either fixed or live cells and tissues that have typically … Read more
Stereo microscopes, in contrast to compound light microscopes, often employ reflected illumination, or light reflected off an object’s surface, rather than transmitted (diascopic) illumination. When a specimen is too thick or opaque for compound microscopy, reflected light from the object enables for investigation. Unlike a compound microscope, transmitted illumination in most stereo microscopes is not … Read more
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