What is Lane-Eynon Method? Principle of Lane-Eynon Method The method based on reduction of cupric ions (Cu²⁺) to cuprous oxide (Cu₂O) by the reducing sugars in alkaline condition. In the reaction, CuSO₄ from Fehling A and alkaline tartrate from Fehling B react to form a deep blue cupric–tartrate complex, which remain stable in hot solution … Read more
What is Gradient PCR? Gradient PCR (G-PCR) refer as a method by which many annealing temperatures are tested at once in one single PCR run. It’s done by special thermal cycler that has gradient heating blocks. In a normal PCR, the temperature of block stay same everywhere. But in gradient PCR, the thermal block is … Read more
Nylander’s Test sometimes also called Bismuth Reduction test, is used for detecting reducing sugars like glucose / fructose etc. The principle is based on reduction of bismuth oxynitrate (BiONO₃) or bismuth subnitrate into metallic bismuth, in alkaline medium. In this reaction, reducing sugar which have free aldehyde or ketone group, they reduce bismuth compound into … Read more
Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.
Picric Acid Test is defined as a very sensitive chemical test, primarily used for detection of reducing sugars in carbohydrates. Picric acid (2,4,6-trinitrophenol) is described as a yellow crystalline solid, the intense yellow being due to its three nitro groups. The principle is that reducing sugars (monosaccharides and some disaccharides) will reduce picric acid, when … Read more
Definition of Stage Micrometer A stage micrometer is a calibrated slide with a ruled scale used to calibrate microscopes and measure the size of objects under a microscope. Principle of Stage micrometer Parts of Stage micrometer Types of Stage Micrometer A. Based on Scale Design / Measurement Function B. Based on Physical Construction/ Traceability C. … Read more
Method of Calibrating a Microscope Importance of Microscope Calibration FAQ
Definition of Vortex Mixer A vortex mixer is a laboratory device that uses rapid circular oscillations to create a swirling motion, or vortex, in small volumes of liquid samples for efficient mixing and blending. Principle of Vortex Mixer Parts of Vortex Mixer Operating Procedure of Vortex Mixer To operate a vortex mixer, follow these steps: … Read more
Homogenizer Definition A homogenizer is a device used to mix, emulsify, and evenly distribute particles in liquids, creating a uniform and homogeneous mixture. Principle of Homogenizer Parts of Homogenizer 1. Homogenizing Valve – It is the main part where actual homogenization occur, the liquid is forced by a very small gap creating high velocity & … Read more
Definition of Incinerator An incinerator is a specialized furnace designed for burning potentially dangerous materials in a controlled manner, typically at high temperatures, to eliminate pollutants and safely dispose of waste. Working principle of Incinerator Operating procedure of Incinerator Parts of Incinerator Primary Chamber – It is the main combustion chamber where the waste materials … Read more
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