In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
• Edited by Sourav Pan • • 5 revisions • 14 min read
Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.
What is Seliwanoff’s Test? Seliwanoff’s Test is a fundamental biochemical assay designed to distinguish between aldose and ketose sugars based on their structural differences. Named after chemist Theodor Seliwanoff, this test leverages the principle that ketoses (sugars with a ketone group, like fructose) dehydrate more rapidly than aldoses (sugars with an aldehyde group, like glucose) when heated with a … Read more
A PCR machine is a common tool found in labs, especially if you’ve ever dealt with DNA testing or medical diagnostics. At its core, it’s designed to create millions of copies of a specific DNA segment, which sounds simple but is incredibly powerful for tasks like identifying infections, studying genetic mutations, or even solving crimes. … Read more
What is Electroporator (Electroporation machine)? An electroporator, often called an electroporation machine, is a lab instrument used to temporarily make cell membranes more permeable. Think of it as a precision tool that helps scientists sneak substances like DNA, drugs, or proteins into cells—something that’s tricky to do manually because cell membranes are naturally protective. The … Read more
What is a micropipette? A micropipette is a precision laboratory instrument designed to measure and transfer extremely small volumes of liquid, typically ranging from 0.1 microliters to 1,000 microliters. Commonly used in molecular biology, chemistry, and medical labs, it operates like a highly accurate eyedropper, enabling researchers to handle samples with meticulous control. The device … Read more
What is Chromatography? Chromatography is a powerful laboratory method used to separate, identify, and analyze the components of complex mixtures. Think of it as a molecular race where different substances travel at varying speeds based on their interactions with two key elements: a stationary phase (a solid or liquid-coated surface) and a mobile phase (a … Read more
What is a Spectrophotometer? Definition of Spectrophotometer A spectrophotometer is a laboratory instrument that measures the amount of light absorbed by a sample and is used to determine properties such as concentration or absorption patterns of substances. Principle of Spectrophotometer Types of Spectrophotometer Spectrophotometers are analytical devices for measuring, at designated wavelengths, the intensity of … Read more
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