Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).
What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more
What is Repetitive sequence-based PCR (rep-PCR)? Working Principle of rep-PCR The rep-PCR working principle is based on the occurrence of conserved repetitive DNA elements (REP, ERIC, BOX, GTG5 etc.), which are dispersed at multiple sites within prokaryotic genomes, and these elements serve as priming sites for amplification. By the use of specifically designed primers, annealing … Read more
What is Nanoparticle-Assisted PCR (nanoPCR)? Working Principle of Nanoparticle-Assisted PCR (nanoPCR) The nanoPCR method is introduced with nanoparticles (NPs) being added into a conventional PCR mixture which contains template DNA, primers, DNA polymerase, dNTPs, and buffer. With optimal NP size (often <100 nm) and concentration being selected, thermal conductivity is enhanced by NPs which accelerates … Read more
Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR 2. High-fidelity PCR 3. Hot-Start PCR 4. Fast-cycling PCR 5. Touch down (TD) PCR 6. Real-Time PCR (qPCR) 7. Digital PCR (dPCR) 8. High-Resolution Melt (HRM) PCR 9. Reverse-Transcriptase PCR (RT-PCR) 10. RT-qPCR – Real-time quantitative reverse transcription … Read more
In molecular biology Western blotting is a rapid and sensitive assay for detection and characterization of proteins. This technique exploits the inherent specificity of antigen-antibody interaction to identify specific antigens by polyclonal or monoclonal antibodies.
• Edited by Sourav Pan • • 5 revisions • 14 min read
Brucella is an intracellular parasite which causes epizootic abortions for animals, and septicemic febrile disease or localized infections of tissues, bones or organ systems of humans. Brucella species are very meticulous and require a rich nutrient environment to develop. Additionally, Brucella species are highly infectious, and therefore extreme caution should be exercised when handling.
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.
What is Seliwanoff’s Test? Seliwanoff’s Test is a fundamental biochemical assay designed to distinguish between aldose and ketose sugars based on their structural differences. Named after chemist Theodor Seliwanoff, this test leverages the principle that ketoses (sugars with a ketone group, like fructose) dehydrate more rapidly than aldoses (sugars with an aldehyde group, like glucose) when heated with a … Read more
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