SXT (Sulfamethoxazole-Trimethoprim) Test – Principle, Procedure, Result
The SXT (sulfamethoxazole-trimethoprim) susceptibility test tells the difference between group A and B streptococci and other hemolytic streptococci.
Practical
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Heat Coagulation Test of Proteins – Principles, Procedure, Result
agulation of proteins is a test in biochemistry to find whether there are proteins such as albumin and globulin found in proteins. The coagulation of proteins in reaction the heat stimulus is an typical phenomenon. The process of coagulation by heat of proteins happens in two phases; denaturation or Agglutination, or the dissociation of the protein denatured in the form it is in.
Millon’s Test -Principle, Procedure, Result, Uses
illon’s test can be described as an analytical test for the identification that the amino acid tyrosine which is the sole amino acid with the phenolic group.
Bial’s Test- Principle, Procedure, Result, Uses
Bial test is a biochemical test which is used for the detection of pentose sugars and to differentiate them from hexose sugars. It is mainly employed for identifying sugars like ribose and xylose and is also useful for the detection of RNA because RNA contains ribose sugar. This test is based on the reaction of … Read more
Beta (β) Lactamase Test – Principle, Procedure, Results
Many bacteria produce a group of enzymes known as beta-lactamases that are mediated through genes in plasmids or the chromosomes. Beta-lactamase can be a constant process or it may be triggered through contact with antimicrobials. Beta-lactamases hydrolyze (and consequently inhibit) the beta-lactam ring of many Cephalosporins as well as penicillins. Beta-Lactamase Test is a rapid test to determine the presence of beta-lactamase , an enzyme that is produced by the strains from Staphylococcus aureus Neisseria gonorrhoeae, Branhamella catarrhalis as well as Haemophilus influenzae.
Nitrocefin Test (Beta-Lactamase Test) – Principle, Procedure, Uses
The nitrocefin biochemical test is a sensitive technique for detecting beta-lactamase-producing strains of N. gonorrhoeae, H. influenzae, Staphylococcus spp, Enterococcus spp, and Moraxella (Branhamella) catarrhalis. Nitrocefin is the only reliable test for detecting beta-lactamase-producing Enterococcus spp.
Mucic acid test – Principle, Procedure, Result, Uses
Mucic acid test is a specific chemical test used for the identification of galactose and galactose containing sugars like lactose. It is also referred to as galactaric acid test. It is based on the oxidation reaction of sugars with concentrated nitric acid. In this test the sugar sample is heated with concentrated nitric acid which … Read more
Molisch Test – Objective, Principle, Procedure, Result
This is a test that is common for all carbohydrate larger than the tetroses. The test works on the basis that pentoses as well as hexoses can be dehydrated using conc. sulfuric acid, resulting in furfural or hydroxyl-methyl furfural or hydroxyl methyl furfural. These compounds condense with a-naphthol to create a purple condensation product. Molisch’s … Read more
Rapid Furfural Test for Glucose and Fructose – Principle, Procedure, Result
Rapid Furfural Test is a qualitative chemical test used to differentiate ketohexoses from aldohexoses based on the rate of dehydration reaction. It is mainly used for the identification of fructose and other ketose sugars in the presence of aldose sugars like glucose. It is based on the principle that ketohexoses are dehydrated rapidly when heated … Read more
Fehling’s Test – Principle, Procedure, Result
This test also can differentiate between ketone functional groups and water-soluble carbohydrates.