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Esculin Hydrolysis test – Principle, Procedure, Result, Uses

Esculin Hydrolysis test Principle, Procedure, Result

The Esculin hydrolysis test is a biochemical test which is used for the identification and differentiation of certain bacteria, mainly Enterococcus species and Group D streptococci from other streptococci. It is also used for identifying some other organisms such as Listeria species and members of Bacteroides fragilis group. It is based on the ability of … Read more

Bile Solubility Test – Principle, Procedure, Result, Uses

Bile Solubility Test - Principle, Procedure, Result

It is a biochemical test used in microbiology for the identification of Streptococcus pneumoniae from other alpha hemolytic streptococci. It is mainly based on the ability of pneumococcal cells to undergo lysis in presence of bile salts. This test is commonly performed in diagnostic laboratories for confirmation of pneumococcal isolates. It is the process in … Read more

Leucine aminopeptidase (LAP) Test – Principle, Procedure, Results

Leucine aminopeptidase (LAP) Test Principle, Procedure, Results

Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).

Lipid Hydrolysis Test – Principle, Procedure, Result

Lipid Hydrolysis Test Principle, Procedure, Result

It is a biochemical test that is used to determine the ability of microorganism to produce and secrete the enzyme lipase. It is also referred to as lipase test. The complex lipids such as triglycerides are large molecules and these cannot pass through the bacterial cell membrane. Due to this reason some bacteria releases the … Read more

Ouchterlony Double Immunodiffusion Method

Ouchterlony Double Immunodiffusion Method

Ouchterlony Double Immunodiffusion Method is a qualitative immunological technique that is used to study antigen–antibody reaction in vitro. It is the process in which both antigen and antibody are allowed to diffuse freely in a semi-solid medium usually agar or agarose gel. This method was developed by Örjan Ouchterlony and it is commonly used for … Read more

Arneth Count for Neutrophils – Principle, Procedure, Result, Uses

Arneth Count for Neutrophils: Principle, Procedure and Clinical Significance

The Arneth count is a hematological method used for the classification of neutrophils based on the number of lobes present in the nucleus. It is the process in which neutrophils are grouped into different classes according to nuclear segmentation and this helps in understanding the age and functional status of the cells. In this method … Read more

BOD Incubator – Principle, Parts, Procedure, Application

BOD Incubator - Definition, Principle, Components, Procedure, Application

A BOD incubator is a laboratory instrument that is used for maintaining a controlled temperature environment required for the estimation of Biochemical Oxygen Demand of water samples. It is designed to operate both at heating and cooling conditions which is different from ordinary incubators. It is mainly adjusted at a fixed temperature of 20°C which … Read more

Lipase Test – Principle, Procedure, Results, Uses

Lipase Test Principle, Procedure, results

A lipase test is a laboratory diagnostic test which is done to measure the level of lipase enzyme in the blood. Lipase is a digestive enzyme which is mainly produced by pancreas and it helps in digestion of fats. It is the process in which dietary triglycerides are broken down into fatty acids and glycerol … Read more

Malonate Test – Principle, Procedure, Results, Uses

Malonate Test Principle, Procedure, Results

The purpose of the malonate test is to see if the organism can get all of its energy from malonate.

Microdase Test – Principle, Procedure, Result, Uses

Microdase Test Principle, Procedure, Result

The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.

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