Counter current immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody migrate towards opposite directions and form a visible white precipitate in the area between the wells.
Rocket Immunoelectrophoresis (RIEP) also known as electro-immuno diffusion is a simple, quick and reproducible method for determining the concentration of antigen (Ag) in an unknown sample.
It is a type of biochemical test, which is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase).
Spread Plate Method in microbiology The spread plate method is used to isolate individual colonies from a diluted sample of mix population. Different methods have been developed for the isolation of microorganisms from a mixture sample but among them Spread plate culture method is the most widely used technique in laboratory. An ideal spread plate … Read more
The pour Plate Method technique was created in the laboratory by Robert Koch and is still widely used in the laboratory since his time. This technique is appropriate for Microaerophilic, facultative, or anaerobic microorganisms. It is straightforward to use, requires fewer resources, and is simple and cost-effective. However, it requires the specimen to be placed … Read more
What is Nitrate Reduction Test? Purpose of Nitrate Reduction Test Principle of Nitrate Reduction Test Reactions of Nitrate Reduction Test Use of Zinc powder Use of A Durham tube Requirements Composition of Nitrate broth (medium) Ingredients Gms / Litre Peptic digest of animal tissue 5.000 Meat extract 3.000 Potassium nitrate 1.000 Sodium chloride 30.000 Final … Read more
IMViC is a mnemonic that stands for four tests used in the identification of enteric bacteria: Indole, Methyl Red, Voges-Proskauer, and Citrate. These tests are commonly used in microbiology laboratories to identify bacterial species in the Enterobacteriaceae family, which includes many common pathogens such as Escherichia coli and Salmonella. The principle behind the IMViC tests … Read more
The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.
About Simmons citrate agar test Simmons citrate agar test helps in detection of citrate utilizing bacteria. In this test, a specific medium is used called Simmons’ citrate agar which helps to differentiate among various Gram-negative enteric bacilli. Simmons’ citrate agar is a type of selective and differential medium. Which is used for the study of … Read more
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
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