What is Auramine- Rhodamine Staining? Principle of Auramine- Rhodamine Staining Auramine and Rhodamine are nonspecific fluorochrome dyes that are attracted to acid-fast microorganisms. In the case of Mycobacteria, the dyes can selectively bind to the mycolic acid present in the cell wall, allowing the stain to penetrate. This compound is resistant to acid-alcohol decolorizer solutions. … Read more
Wright’s stain is a modified Romanowsky stain. In hematology laboratory, it is used for the staining of peripheral blood smear, bone marrow aspirates, and urine samples.
Different stains react or concentrate on different areas of a tissue or cell These properties can be utilized to highlight certain areas or regions. A few of the most well-known biological staining methods can be found below. If not otherwise indicated All of these dyes can be used on tissues and cells that are fixed as well as essential dyes (suitable for use in live organisms) are indicated.
Different stains have been developed over time to distinguish bacteria species, separating them morphologically and the specific characteristics they possess. The most popular stain is Gram staining, acid-fast staining, and endospore staining. Each stain aims at identifying and defining bacteria according to their forms and morphologies.
Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. Some … Read more
Aim To distinguish between living and dead cells. Principle The viability Staining Method mainly used to detect living and dead cells in culture. This method is based on the fact that, certain changes take place in dead cells which result in these cells giving a different reaction to stains from that of living cells. Requirement … Read more
Flow cytometry is a fast and stable method for the quantification of viable cells. Determining cell viability is a significant step when assessing a cell’s reply to medication or other environmental agents. It is additionally essential to recognize dead cells in a cell suspension in order to eliminate them from the study. Dead cells can … Read more
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