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IMViC Test – Principle, Procedure, Result, Uses, Examples

Operating Procedure of Fluorescence spectroscopy

What is IMViC test? The IMViC test is a group of biochemical tests which is used for the identification and differentiation of bacteria belonging to the family Enterobacteriaceae. It is mainly used to distinguish fecal coliform bacteria from non-fecal coliform bacteria. The term IMViC is an acronym which stands for Indole, Methyl Red, Voges–Proskauer and … Read more

Iodine Test – Principle, Procedure, Result, Uses

Iodine Test - Iodine test for starch Principle, Procedure, Result, Uses

Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.

Affinity chromatography – Principle, Types, Steps, Applications

Affinity chromatography Principle, Types, Steps, Applications

Affinity chromatography is a type of liquid chromatography technique which is used for separation and purification of biomolecules based on a highly specific and reversible interaction between the target molecule and a specific ligand. It is the process where the target molecule selectively binds with a ligand that is immobilized on a solid matrix. This … Read more

Bench Top Centrifuge – Principle, Procedure, Types, Parts, Uses

Bench Top Centrifuge - Principle, Types, Parts, Uses

A bench top centrifuge is a laboratory instrument which is used for separation of components present in a liquid sample based on their density. It is a compact device that is designed to be placed on laboratory bench and is commonly used in routine laboratory work. It is the process in which centrifugal force is … Read more

Hematocrit Centrifuge -Principle, Procedure, Parts, Uses

Hematocrit Centrifuge - Definition, Principle, Parts, Uses

A hematocrit centrifuge is a laboratory instrument which is used to determine the hematocrit value of blood. It is the percentage volume of red blood cells present in the total volume of whole blood and this value is also referred to as packed cell volume (PCV). It is commonly used in hematology laboratories for routine … Read more

Simmons Citrate Agar – Principle, Composition, Procedure, Result, uses

Simmons citrate agar test

Simmons Citrate Agar is a chemically defined medium in which sodium citrate act as the only carbon source and ammonium dihydrogen phosphate act as the only nitrogen source. It is the medium developed as a modification of Koser’s citrate medium and it is used mainly to differentiate members of Enterobacteriaceae. It is the process in … Read more

Gelatin Hydrolysis Test – Purpose, Principle, Procedure, Result.

Gelatin Hydrolysis Test Purpose, Principle, Procedure, Result.

The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.

CAMP Test – Principle, Procedure, Result, Uses

Christie, Atkins, and Munch-Peterson (CAMP) test

CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.

Reverse CAMP test – Principle, Procedure, Result, Uses

Reverse CAMP test

In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.

Biuret Test For Protein – Principle, Procedure, Result, Uses

Biuret Test For Protein Principle, Procedure, Result, Application.

Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.

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