Practical

UV-Vis Spectroscopy, short for Ultraviolet-Visible Spectroscopy, is an analytical technique widely used in the fields of chemistry, physics, biochemistry, and molecular biology. It’s based on the absorption or transmission of UV and visible light by substances. At its core, UV-Vis Spectroscopy involves shining ultraviolet or visible light through a sample and measuring how much of … Read more

UV Spectroscopy is a scientific method that’s like a detective tool for scientists. It helps them understand what’s happening in a molecule when it interacts with light, specifically ultraviolet light. This type of light is invisible to our eyes but plays a crucial role in the world of molecules. Imagine a molecule as a tiny, … Read more

What is Objective Lens? Purpose of Objective Lenses Objective lenses serve several key purposes in optical instruments, playing a critical role in magnification, image formation, and resolution. The primary purposes of objective lenses include: Specification of Objective Lens The specification of an objective lens encompasses several key parameters that define its performance and suitability for … Read more

A microscope condenser is an integral optical component in the realm of microscopy, designed to focus and direct light onto the specimen being examined. This specialized lens system is strategically positioned beneath the microscope stage, a placement crucial for optimal illumination. The primary function of the condenser is to gather light from the microscope’s light … Read more

What are Microscopes? Parts of a Microscope The microscope parts are divided into two main categories,  such as; Structural parts of a microscope and their functions Structural parts of a microscope Location Function Head (Body Tube) Situated at the microscope’s apex. Serves as a pivotal connector, linking the eyepiece to the objective lenses, ensuring a … Read more

Ion exchange chromatography (IEC) is a chromatographic technique that allows for the separation of ions and polar molecules based on their charge. It utilizes a stationary phase composed of an insoluble matrix with covalently bonded charged groups. These charged groups can attract and bind ions of the opposite charge present in the mobile phase, which … Read more

What is Ocular Micrometer? Working Principle of Ocular Micrometer The principle of an ocular micrometer revolves around using a calibrated scale to accurately measure the size of objects viewed through a microscope. This scale, typically divided into 100 divisions, each measuring 0.01 millimeters (µm), is an essential component placed within the eyepiece of the microscope. … Read more

What is Toolmakers Microscope? Principle of Toolmakers Microscope The working principle of the toolmakers microscope relies on the principles of optics, similar to a conventional light microscope. The microscope is equipped with a light source that directs light upward, focusing it onto the object being observed. When the light falls on the object, it casts … Read more

What is Gradient PCR? Definition of Gradient PCR Gradient PCR is a specialized molecular technique that allows for the simultaneous testing of different annealing temperatures in a single PCR run, optimizing the conditions for precise DNA amplification. Principle of Gradient PCR The principle of Gradient PCR revolves around the capability to simultaneously evaluate a range … Read more