10 min read 
Principle of Nitrite Reduction Test Nitrites combine with an acidic solution of sulfanilic acid and alpha-naphthylamine to produce a red azo dye. In each test reaction, the appearance of the red dye shows the presence of NO2- in the test tube, either as an unreduced main substrate, a product of the test organism’s reduction of … Read more
8 min read 
Test Name Esculin Hydrolysis test Purpose This test is used to try to figure out what kind of Enterobacteriaceae it is and how it differs from other kinds.To see if the microbe can use esculin as a source of carbon by breaking it down with water. Media Required Esculin Hydrolysis Agar Result Positive: Blackened medium, … Read more
3 min read 
Purpose of Gram-Sure Test Principle of Gram-Sure Gram-negative organisms’ cell walls include aminopeptidase, which can be identified through the hydrolysis of L-alanine-7-amido-4-methylcoumarin, a nonfluorescent substrate, into a fluorescent product. Materials Required Quality Control All Gram-Sure lot numbers were tested with the following quality control organisms and determined to be acceptable. The examination of control organisms … Read more
14 min read 
Test Name Deoxyribonuclease (DNase) Test Detection DNases enzyme Uses To find out if an organism can break down DNA and use it to get carbon and energy so it can grow.To distinguish Staphylococcus aureus from other Staphylococci. Result DNase Test Agar: After adding 1N HCl, a clear zone forms around the growth line, but the … Read more
9 min read 
Cetrimide Selective Agar is a solid medium that is recommended for use in qualitative procedures for selective isolation and presumed identification of Pseudomonas aeruginosa and other nonfermenting, gram-negative bacilli.
7 min read 
Test Name Casein Hydrolysis Test Detection Enzyme caseinase producing microorganisms Uses To show that some microorganisms can make enzymes outside of their cells that can break down the milk protein casein. Result A clear, transparent zone can be seen around the area where the colonies are growing, or sometimes under the area where the colonies … Read more
6 min read 
Overview of Butyrate Test Test Name Butyrate Disk Test Detection Enzyme butyrate esterase Uses This is a quick way to find out if Moraxella catarrhalis has the enzyme butyrate esterase. Result A blue colour development Require Bromochloro-indolyl butyrate and 4-methylumbelliferyl impregnated discs Test Organism Moraxella catarrhalis ATCC 25240: butyrate positive Butyrate Disk Test Purpose Principle … Read more
7 min read 
Test Name Acetamide utilization Test Purpose Differentiate microorganisms according to their capacity to utilise acetamide as their only carbon source. Microorganisms Tested Isolated colonies of Gram-negative, non-glucose-fermenting rods that emit a luminous pigment and are suggestive of P. aeruginosa. Result Positive: The slant shows growth, and its colour changes from green to intense blue.Negative: There … Read more
18 min read 
What is API (Analytical Profile Index) 20E Test? Principle of APT Test for Bacteria API 20E Test Kit API 20E is a specialized kit designed for bacterial identification, consisting of 20 distinct tests. These tests aid in characterizing various metabolic activities and enzymatic reactions of the organisms being examined. Each test provides valuable information to … Read more
4 min read 
The oxidase enzyme is a key factor in distinguishing Staphylococcus from Micrococcus, and the Microdase Disk is a reagent-impregnated disc recommended for use in qualitative procedures.
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