IMViC test is a group of four biochemical tests which are used in microbiology laboratory to identify and differentiate Gram-negative bacteria especially coliform group and Enterobacteriaceae family. These tests are commonly performed after Gram staining and the reactions are stable and used as important identification tests. IMViC is an acronym in which I stands for
Iodine testing is a chemical test that distinguishes mono- or diaccharides from polysaccharides such as amylase, glycogen, and dextrin. Starch-iodine is a variant of this test. It’s used to determine if there is glucose in the leaves.
The gelatin proteins derived from the animal connective tissue, collagen. Gelatin is produced when collagen is boiled in water. A gelatin hydrolysis test is used to detects the presence of gelatinases. Gelatinases are extracellularly secreted by some bacteria which hydrolyze or digest gelatin.
CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.
Learn the reverse CAMP test principle, procedure and result, including bow-tie hemolysis in Clostridium perfringens, positive organisms, uses and CAMP test differences.
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.
It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.
The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .
Bacillus thuringiensis (Bt) is a Gram positive bacteria. It is a spore forming bacteria. It is found naturally in different environment like soil water dead insects and plant surface. It is known for producing special protein crystal during sporulation phase. These protein crystal is known as delta endotoxin (Cry and Cyt proteins). When these crystal
Learn Nylander’s test for reducing sugars, including its principle, reagent composition, procedure, black bismuth result, interpretation, limitations, and modern relevance.
