What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more
What is Repetitive sequence-based PCR (rep-PCR)? Working Principle of rep-PCR The rep-PCR working principle is based on the occurrence of conserved repetitive DNA elements (REP, ERIC, BOX, GTG5 etc.), which are dispersed at multiple sites within prokaryotic genomes, and these elements serve as priming sites for amplification. By the use of specifically designed primers, annealing … Read more
What is Nanoparticle-Assisted PCR (nanoPCR)? Working Principle of Nanoparticle-Assisted PCR (nanoPCR) The nanoPCR method is introduced with nanoparticles (NPs) being added into a conventional PCR mixture which contains template DNA, primers, DNA polymerase, dNTPs, and buffer. With optimal NP size (often <100 nm) and concentration being selected, thermal conductivity is enhanced by NPs which accelerates … Read more
Types of PCR Here is the list of Types of PCR based on different categories; 1. Conventional PCR 2. High-fidelity PCR 3. Hot-Start PCR 4. Fast-cycling PCR 5. Touch down (TD) PCR 6. Real-Time PCR (qPCR) 7. Digital PCR (dPCR) 8. High-Resolution Melt (HRM) PCR 9. Reverse-Transcriptase PCR (RT-PCR) 10. RT-qPCR – Real-time quantitative reverse transcription … Read more
A PCR machine is a common tool found in labs, especially if you’ve ever dealt with DNA testing or medical diagnostics. At its core, it’s designed to create millions of copies of a specific DNA segment, which sounds simple but is incredibly powerful for tasks like identifying infections, studying genetic mutations, or even solving crimes. … Read more
What is Electroporator (Electroporation machine)? An electroporator, often called an electroporation machine, is a lab instrument used to temporarily make cell membranes more permeable. Think of it as a precision tool that helps scientists sneak substances like DNA, drugs, or proteins into cells—something that’s tricky to do manually because cell membranes are naturally protective. The … Read more
What is a micropipette? A micropipette is a precision laboratory instrument designed to measure and transfer extremely small volumes of liquid, typically ranging from 0.1 microliters to 1,000 microliters. Commonly used in molecular biology, chemistry, and medical labs, it operates like a highly accurate eyedropper, enabling researchers to handle samples with meticulous control. The device … Read more
What is Chromatography? Chromatography is a powerful laboratory method used to separate, identify, and analyze the components of complex mixtures. Think of it as a molecular race where different substances travel at varying speeds based on their interactions with two key elements: a stationary phase (a solid or liquid-coated surface) and a mobile phase (a … Read more
What is a Spectrophotometer? Definition of Spectrophotometer A spectrophotometer is a laboratory instrument that measures the amount of light absorbed by a sample and is used to determine properties such as concentration or absorption patterns of substances. Principle of Spectrophotometer Types of Spectrophotometer Spectrophotometers are analytical devices for measuring, at designated wavelengths, the intensity of … Read more
What is Single Use Bioreactor? Working Principle of Single Use Bioreactor Parts of Single Use Bioreactor Comprising numerous essential components meant to enable effective and sterile biological operations, a single-use bioreactor (SUB) Types of Single Use Bioreactor Offering disposable, pre-sterilized systems that cut turn-around times and eliminate the need for cleaning, single-use bioreactors (SUBs) have … Read more
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