Plant Tissue Culture – Definition, techniques, Media composition, types, application.

Plant tissue culture

What is Plant Tissue Culture? Plant tissue culture Definition Plant tissue culture is a biotechnology technique that involves growing plant cells, tissues, or organs in a controlled, sterile environment on a nutrient medium. This method exploits the totipotency of plant cells, allowing for the rapid propagation of plants, production of disease-free specimens, and conservation of … Read more

Northern Blotting – Protocol, Principle, Application, Result

Northern Blot Protocol, Principle, Application, Result

Northern blotting or Northern hybridization is a widely used technique in molecular biology to determine the molecular weight of mRNA and to measure relative amounts of mRNA present in different samples and for identifying alternatively spliced transcripts and multigene family members.

Measurement Of Microorganisms – Micrometry

Measurement Of Microorganisms - Micrometry

A microscope comes with an objective of 10X (low power) 45X (high power) and 100X (oil immersion). and the ocular lens with 5X, 10X and 15X. In general, an 10-X ocular lens is used in most cases. If an animal can be clearly seen with 10X ocular lens and 45X objective, this means that the size is 10×45, which is 450 times larger than the standard one. We don’t know the exact measurement is ? It could be determined using an calibrated scale. Thus the calibration of all lenses (eyepieces) is performed.

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