Sourav Pan

What is Reversed-Phase Chromatography? Principle of Reversed-Phase Chromatography The principle of reversed-phase chromatography (RPC) is rooted in the interaction between molecules possessing hydrophobic groups. In RPC, the stationary phase comprises a solid support material that is modified with both hydrophobic and hydrophilic groups. When a sample is introduced into the chromatographic system, the solvent molecules … Read more

What is Liquid Chromatography? Types of Liquid Chromatography Liquid chromatography can be classified into four main types based on the components involved in the chromatographic process: These different types of liquid chromatography offer versatility in separating and analyzing diverse compounds, catering to various research and industrial applications. Researchers select the appropriate type of liquid chromatography … Read more

What is Hydrophobic Interaction Chromatography (HIC)? Principle of Hydrophobic Interaction Chromatography (HIC) The principle of Hydrophobic Interaction Chromatography (HIC) is based on the interactions between the hydrophobic regions of sample protein molecules and a hydrophobic stationary phase or matrix. This principle allows for the separation and purification of proteins based on their hydrophobicity. In HIC, … Read more

What is Flash chromatography? Principle of Flash column chromatography The principle of flash chromatography revolves around the rapid movement of the eluent, a liquid, through a short glass column under gas pressure, typically nitrogen or compressed air. The glass column is packed with a stationary phase consisting of an adsorbent material with a defined particle … Read more

What is Chromatography? Chromatography Definition Chromatography is a technique used to separate and analyze the components of a mixture based on their properties and interactions with a stationary and mobile phase. What is a stationary phase? What is the mobile phase? Types of Chromatography Here is the Types of Chromatography; Type of Chromatography Description Gas … Read more

What is Two-Dimensional (Crossed) lmmunoelectrophoresis? Principle of Two-Dimensional (Crossed) lmmunoelectrophoresis The principle of Two-Dimensional (Crossed) Immunoelectrophoresis involves the combination of two distinct electrophoretic steps to separate and identify proteins based on their charge, size, and antigen-antibody interactions. The technique consists of the following steps: The principle of Two-Dimensional Immunoelectrophoresis exploits the migration characteristics of proteins … Read more

What is Immunoelectrophoresis? Immunoelectrophoresis is a biochemical approach for protein separation and characterization that is based on electrophoresis and antibody-antigen interactions. It uses immunodiffusion and electrophoresis techniques to identify and analyse specific antigens within a protein mixture. The immunoelectrophoresis procedure begins with electrophoresis, which separates the antigen mixture into its distinct components. This is accomplished … Read more

What is Polyacrylamide Gel Electrophoresis (PAGE)? Definition of Polyacrylamide Gel Electrophoresis (PAGE) Polyacrylamide Gel Electrophoresis (PAGE) is a technique used to separate and analyze proteins or nucleic acids based on their mobility in a porous polyacrylamide gel matrix under the influence of an electric field. Types of Polyacrylamide Gel Electrophoresis (PAGE) There are several types … Read more

What is SDS-PAGE? Definition of SDS-PAGE SDS-PAGE is a technique used to separate proteins based on their molecular weight by using a gel matrix and the denaturing agent sodium dodecyl sulfate (SDS). Overview of SDS PAGE SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a popular method for studying protein mixtures. By sorting proteins depending on … Read more

What is Mass Spectrometry (MS)? Definition of Mass Spectrometry (MS) Mass Spectrometry (MS) is an analytical technique used to identify the chemical composition of a sample by measuring the mass-to-charge ratio of its ions, resulting in a mass spectrum that reveals the molecular and structural information of the sample. Mass Spectrometry Principle Mass Spectrometry operates … Read more