What is Thayer Martin Medium? Composition of Martin Lewis Agar Ingredients Gms/L Casein Peptone 7.5 Meat Peptone 7.5 Corn Starch 1.0 Potassium Phosphate, dibasic 4.0 Potassium Phosphate, monobasic 1.0 Sodium Chloride 5.0 Agar 10.0 Hemoglobin 10.0 Dextrose 1.5 Enrichment Bio-X 10 ml Antibiotics V.C.A.T 10 ml pH 7.2 +/- 0.2 at 25º C Enrichment Bio-X … Read more
Peptone Water is a type of broth medium. This media is mainly used to grow the organism and is also used as a base for determining carbohydrate fermentation patterns of non-fastidious organisms. It is also used to detect indole production by the organism.
Clinical materials containing small numbers of Vibrio should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar (M189). Alkaline Peptone Water is a suitable enrichment broth for this purpose.
production of blood agar after the addition of liquid blood.
Sheep blood agar The principle of sheep blood agar Haemolysins are exotoxins created by bacteria that kill Red blood cell clots. The haemolytic process can be observed on blood Agar Plates. In blood agar plates, colonies of haemolytic bacterium may be covered by a the clear, uncoloured zone, in which the Red blood cells are … Read more
What is Mueller Hinton Agar (MHA)? Why MHA is used for antibiotic susceptibility testing (AST)? Mueller Hinton Agar (MHA) is commonly used for antibiotic susceptibility testing due to several key reasons: Mueller Hinton Agar (MHA) Principle The principle of Mueller Hinton Agar (MHA) revolves around its composition and the controlled levels of various components to … Read more
Salmonella Shigella (SS) Agar can be moderately specific and a differing medium used for the cultivation, isolation as well as differentiation Salmonella spp. and a few varieties that belong to Shigella spp.
What is the Lysine Iron Agar (LIA) Test? The solid medium Lysine Iron Agar (LIA) is indicated for use in qualitative techniques for the differentiation of microorganisms based on the generation of lysine decarboxylase and hydrogen sulphide. Purpose of Lysine Iron Agar (LIA) Test Principle of Lysine Iron Agar (LIA) Test Lysine Iron Agar Composition … Read more
Stokes disc diffusion method isn’t as well-standardized as Kirby-Bauer’s method and is utilized in labs especially where the exact amount of antimicrobial present in discs isn’t known because of the difficulty in getting discs and correctly storing them or when other requirements needed for the Kirby-Bauer method cannot be fulfilled.
Nagler’s Reaction or Lecithinase test is a test in biochemistry used to detect organisms that produce the phospholipases (lecithinases) e.g. Clostridium perfringens. Its alpha (a) toxin from C. perfringens exhibits the phospholipase enzyme activity, which aids in the distinction between C. perfringens and the other Clostridium species that generate the enzyme lecithinase (C.baratti, C.absonum, C.bifermantans, C.sordelli, and C.novyi) through neutralization of lecithin C activity with an antitoxin.
