Southern Blot – Definition, Principle, Steps, Importance
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
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Gerhardt’s test – Principle, Procedure, Result
Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.
Seliwanoff’s Test – Principle, Procedure, Result, Uses
What is Seliwanoff’s Test? Seliwanoff’s Test is a fundamental biochemical assay designed to distinguish between aldose and ketose sugars based on their structural differences. Named after chemist Theodor Seliwanoff, this test leverages the principle that ketoses (sugars with a ketone group, like fructose) dehydrate more rapidly than aldoses (sugars with an aldehyde group, like glucose) when heated with a … Read more
Cell Organelles – Structure, Functions, Diagram
Your body is currently doing a lot of things simultaneously. Your body is sending electrical impulses, pumping blood and filtering urine.
Gram-Negative Bacteria – Structure, Characteristics, Examples
Gram-negative bacteria are a diverse group of microorganisms known for their unique cell structure, which plays a key role in how they interact with the environment and cause infections. Unlike Gram-positive bacteria, Gram-negative bacteria have a thin peptidoglycan layer sandwiched between two membranes. The outer membrane contains lipopolysaccharides (LPS), which can trigger strong immune responses … Read more
Gram Positive bacteria – Definition, Structure, Characteristics, Examples
Gram-positive bacteria are a group of bacteria classified based on how they react to a lab test called the Gram stain. When stained, these bacteria hold onto a purple dye because of their thick, mesh-like cell wall made of peptidoglycan. This sturdy layer acts like a protective shield, unlike Gram-negative bacteria, which have thinner walls … Read more
McIntosh and Fildes’ Anaerobic Jar – Principle, Parts, Procedure
What is McIntosh and Fildes’ Anaerobic Jar? Designed to provide an oxygen-free environment required for growing bacteria unable of surviving in the presence of oxygen, McIntosh and Fildes’ anaerobic jar is a specialist piece of laboratory equipment. Usually built from strong metal or glass, the jar has a firmly closing lid and has input and … Read more
GasPak Anaerobic System – Principle, Parts, Application
The GasPak Anaerobic System is a laboratory method used to grow bacteria that thrive in oxygen-free environments, known as anaerobes. Using throwaway sachets filled with compounds like sodium borohydride and sodium bicarbonate helps to create an anaerobic (oxygen-free) environment more quickly. These molecules combine to generate hydrogen and carbon dioxide when water is introduced. The … Read more
BOD Incubator – Principle, Parts, Procedure, Application
What is BOD Incubator? BOD incubator Definition The BOD (Biological Oxygen Demand) Incubator is a specialized laboratory instrument designed to provide a controlled temperature environment, typically around 20 degrees centigrade, for microbial and biochemical studies, particularly for assessing the oxygen demand of microorganisms in water samples. BOD incubator Principle Operating on a well engineered mechanism, … Read more
Differential Centrifugation – Principle, Protocol, Uses
Differential centrifugation (or the differential velocity method) is one of the most prevalent techniques applied in biochemistry and cell biology regarding the separation of organelles and other subcellular particles according to their sedimentation rates. It is available not only for separate microscopic analyses, but also as a useful technique for preliminary remediation of suspended nonliving … Read more