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When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.
Little, single-celled creatures living practically everywhere are bacteria. They’re prokaryotic, meaning they lack a real nucleus. They differ from eukaryotic cells in that they lack membrane-bound organelles. Shapes vary; spherical (cocci), rod-like (bacilli), spiral (spirilla), or comma-shaped (vibrios). Their survival in hostile environments depends on this diversity. Some twist like corkscrews, designated as spirochaetes. There … Read more
Benedict’s test is a chemical test that is used to test for the presence of reduced sugars within an analytical test. Thus, simple carbohydrates that contain an aldehyde or free ketone functional group are detected using this test. The test is basing itself upon Benedict’s Reagent (also called Benedict’s solution) which is a complex mix of sodium carbonate, sodium citrate, and the pentahydrate of copper(II) Sulfate.
What is Trinocular Microscope? Principle of Trinocular Microscope A trinocular microscope is similar to a binocular microscope but has an additional third eyepiece for a camera. It shines an LED light on the specimen and projects it onto a computer screen through a digital camera. The trinocular microscope is essentially a binocular microscope that focuses … Read more
What is Rickettsia? Small-sized intracellular bacteria with a 0.3 to 2 µm range make up the genus Rickettsia. Rickettsia’s tiny scale made it first confused as a virus. Having DNA and RNA as their genetic material, these bacteria reproduce via binary fission within the cytoplasm of eukaryotic cells. Gram-negative rickettsia bacteria abound in the digestive … Read more
What is Urinary Tract Infection (UTI)? An illness affecting a portion of the urinary tract—that which comprises the kidneys, ureters, bladder, and urethral—is known as a urinary tract infection (UTI). Following are some salient features of urinary tract infections: Types of Urinary Tract Infection (UTI) UTIs can attack various urinary system segments, causing varied symptoms. … Read more
An electron microscope is an instrument which produces an image of a sample by means of a beam of electrons. Optical microscopes use light to form an image, electron microscopes have greater resolution, and can be used to observe objects that are too small to be seen with an optical microscope. In a large variety … Read more
Larval Forms in Protochordates The larval forms of Protochordates, along with the subphylum Cephalochordata (lancelets) and Urochordata (tunicates), are central to the clarification of the phylogeny of Chordata. The larval stages of Protochordates exhibit very typical characteristics provided within all chordates, such as the presence of a notochord. The larval forms of Protochordates are essential … Read more
What is Theta model of DNA Replication? The theta model of DNA replication is a mechanism for DNA replication which occurs in circular DNA molecules, in particular, those of bacteria, and also the mitochondria and chloroplasts. True to its name, this replication process creates an intermediate structure during replication that resembles the Greek letter theta … Read more
What is Codon? Molecular biologists define a codon as three nucleotides of DNA or RNA. Such is said to code for some particular amino acid, but it may also work as a signal in the course of regulation during the synthesis of protein. In brief, life proceeds generation after generation due to complex steps that … Read more
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