Eastern blot – Protocol, Principle, Application
A Molecular Biology Technique is used for the identification of Post Translation Modification mostly carbohydrate Epitopes by transferring onto a membrane and analyzed using a probe.
Protocols
In this category, you will find protocols for different biological experiments.
Methylene Blue Reduction Test
Methylene Blue Reduction Test also known as mbrt test. It is a qualitative test for milk, it used to check the quality of raw and pasteurized milk. The Methylene Blue Reduction Test is based on the fact that in the presence of oxygen the methylene blue solution forms blue color, and it will lose the … Read more
Dot Blot – Protocol, Principle, Definition.
Dot Blot is a simplified technique of western blotting, which is mainly used for the detection of proteins.
Restriction Digest – Protocol, Principle, result
The restriction enzymes cleave the DNA molecule at a specific site or specific nucleotide sequence, is known as recognition sequences or Restriction sites.
Reverse Blood Grouping – Principle, Procedure, Result
Reverse grouping is defined as using the reagent cells with known ABO antigens and testing the patient’s serum for ABO group antibodies.
Catalase Test Principle, Procedure, Result and Application.
The catalase test is a type of biochemical test which is used to detect the production of catalase enzymes in the organism.
Xanthoproteic test – Principle, Procedure, Result, Application
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc. The presence of proteins can be confirmed qualitatively by usign several tests, Xanthoproteic test is one of them. Due to the presence of characteristic side chains in them, certain amino acids exhibit typical color reactions that form the basis for their identification.
Modified Kirby-Bauer Disc Diffusion Method
Modified Kirby-Bauer disc diffusion test is a standard method that can be used as a regular method to assess the susceptibility test to a bacterial isolate in the clinical laboratory.