Reverse CAMP test – Principle, Procedure, Result, Uses
In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.
Practical
Subcategories
Biuret Test For Protein – Principle, Procedure, Result, Uses
Proteins are polymers of amino acids. They are complex organic compounds containing nitrogen, hydrogen, carbon, and oxygen. Proteins are abundant in our everyday food e.g. egg, soya bean, pulses, fish, milk etc.
Tollens’ Test – Definition, Principle, Procedure, Result, Application
It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.
Oxidase Test – Definition, Principle, Procedure, Result, Application
The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .
Negative Staining – Principle, Procedure, Result, Uses
In negative staining method, an acidic dye is used known as India Ink or Nigrosin. When the bacterial cells are exposed to this stain, due to the presence of acidic nature it readily gives up a hydrogen ion (proton) and the chromophore. As a result, the dye becomes negatively charged, now the bacterial cell surface deflects the stain.
Simple Staining – Procedure, Principle, Result
The main purpose of simple staining is to determine the cell shape, size, and arrangement of bacterial cells.
Gram Staining – Principle, Procedure, Results, Uses
Gram-staining used to differentiate between Gram-Positive and Gram-Negative Bacteria.
Chlamydia Under the Microscope – Procedure, Result
Chlamydia is caused by Chlamydia trachomatis which is a Gram-negative bacterium, and it is the organism that behave as an obligate intracellular parasite because it cannot produce its own metabolic energy. It is the process where the bacterium must survive and replicate inside the eukaryotic host cell, and this organism shows a biphasic developmental cycle … Read more
Hematoxylin and Eosin (H&E) Staining – Principle, Procedure, Result, Uses
Hematoxylin and Eosin (H&E) stain is the routine staining method used in histology and histopathology for observing the basic structure of tissues. It is the process in which two dyes are applied on colorless tissue sections so that the cellular parts become clearly visible under the microscope. Hematoxylin behaves as a basic dye and it … Read more
Acid Fast Stain – Principle, Reagents, Procedure, Result
Acid fast stain is a differential staining method that is used to identify the acid fast bacteria which retain the primary dye even after treatment with acid alcohol. It is the process where the organisms having a high amount of mycolic acids and waxes in their cell wall show resistance to decolourisation. These are hydrophobic … Read more