Culture Media Examples Components/Comments Primary Purpose Bile esculin agar (BEA) Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria. Differential isolation and presumptive identification of group D streptococci and enterococci Bile esculin azide agar with vancomycin Contains azide to inhibit … Read more
When it comes to culturing bacteria, it is crucial to create the same environmental and nutritional conditions as those found in the natural environment. The majority of culture mediums contain water, which is a major source of carbon and energy and nitrogen. It also contains trace elements, as well as some growth factors. In addition, the pH as well as oxygen tension and Osmolarity must also be considered. Some of the components of media for culture include: Although tap water is appropriate for the use of culture media, it shouldn’t be used in the event that it contains a significant amounts of minerals. In these instances the use of demineralised or distillated water is recommended.
Benedict’s test is a chemical test that is used to test for the presence of reduced sugars within an analytical test. Thus, simple carbohydrates that contain an aldehyde or free ketone functional group are detected using this test. The test is basing itself upon Benedict’s Reagent (also called Benedict’s solution) which is a complex mix of sodium carbonate, sodium citrate, and the pentahydrate of copper(II) Sulfate.
An electron microscope is an instrument which produces an image of a sample by means of a beam of electrons. Optical microscopes use light to form an image, electron microscopes have greater resolution, and can be used to observe objects that are too small to be seen with an optical microscope. In a large variety … Read more
What is Baran Pipette Column? Principle of Baran Pipette Column Principles of adsorption chromatography are used in the Baran pipette column. In this technique, the separation of compounds is achieved through their differential interactions with two key phases: in the stationary phase and the mobile phase. The pasteur pipette is packed with a stationary phase, … Read more
By Sourav Pan 4 revisions 45 min readby Sourav Pan
What is an autoclave? An autoclave is a device which finds applications in numerous scientific and medical institutions. Its work principle lies in using the pressure of steam for the cleansing of equipment and materials. During the autoclaving process, the following kinds of germs are killed: bacteria, viruses, and spores. The basic idea of autoclave … Read more
What is gas chromatography? Gas chromatography (GC) is a sophisticated technique that separates, identifies, and quantifies chemical components in complex mixtures. It operates on the principle of distributing the components between a mobile phase and a stationary phase. In GC, the mobile phase is an inert gas like helium, and it acts as a carrier … Read more
What is Laboratory Incubator? An incubator serves as a temperature-controlled, insulated container that laboratories utilise for cultivating and maintaining various biological cultures. This essential piece of equipment creates optimal conditions necessary for growing microorganisms in artificial environments. Like most sophisticated lab instruments, it maintains precise control over environmental factors such as temp, moisture levels, and … Read more
What is Mohr Pipette (graduated pipette)? Parts of Mohr Pipette (graduated pipette) A Mohr pipette, widely used in laboratories for liquid measurement, consists of several components designed to enable accurate and efficient operation. Below are the main parts and their functions: How to Use a Mohr Pipette (graduated pipette) A Mohr pipette is commonly used … Read more
What is Pipette Dropper? Components of Dropper Pipettes A dropper pipette consists of several key components, each serving a specific purpose in the process of transferring small amounts of liquid. Understanding the function of these parts is crucial for ensuring accurate and efficient liquid dispensing. Types of Dropper Pipettes Dropper pipettes come in several variations, … Read more
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