Simmons citrate agar test – Procedure, Principle, Result, Example.

Simmons citrate agar test

About Simmons citrate agar test Simmons citrate agar test helps in detection of citrate utilizing bacteria. In this test, a specific medium is used called Simmons’ citrate agar which helps to differentiate among various Gram-negative enteric bacilli. Simmons’ citrate agar is a type of selective and differential medium. Which is used for the study of … Read more

Total White Blood Cell (WBC) Count – Total Leucocyte Count (TLC)

Total White Blood Cell (WBC) Count - Total Leucocyte Count (TLC)

The White Blood Cells (WBCs) also known as Leucocytes are round nucleated cells in blood, that provide protection against foreign invaders. There are many types of White Blood cells found in blood, and identified as Granulocytes (Neutrophils, Eosinophils & Basophils) and Agranulocytes (Monocytes and Lymphocytes). The size of White Blood Cells can vary between 10 … Read more

Centrifuge Blood Sample – Purpose, Steps, Techniques

Centrifuge Blood Sample - Purpose, Steps, Techniques

What are the components of blood? Blood is a fluid tissue that circulates throughout the body and performs a number of important functions. It is made up of several components, including cells and plasma. Cells: Plasma: Plasma is the liquid portion of the blood that carries the cells and other substances throughout the body. It … Read more

Reticulocyte Count – Principle, Procedure, Calculations and Clinical Significances

Reticulocyte Count : Principle, Procedure, Calculations and Clinical Significances

What is Reticulocytes? Reticulocytes are immature red blood cells (erythrocytes) that have not yet completed the final stages of maturation. They are usually produced in the bone marrow and have a characteristic reticulated (network-like) appearance when stained with special dyes. Reticulocytes are important in the evaluation of anemia, as they provide information about the bone … Read more

Total Red Blood Cell (RBC) Count – Procedure, Principle, result

Total Red Blood Cell (RBC) Count - Procedure, Principle, Result

Haemocytometry is a quantitative method that quantifies the RBC count in blood samples. The manual method of counting RBCs is the Haemocytometer (or Neubauer’s chamber slides). Modern devices, such as photometric and electrometric counters, can be more precise or automated. They can count the cells in the blood sample. It is impossible to count the … Read more

Sahli’s Method For The Estimation Of Hemoglobin

Sahli’s Method For The Estimation Of Hemoglobin

Hemoglobin, often abbreviated as Hb, is a vital protein found in red blood cells (RBCs). Its primary role in the human body includes the transport of oxygen from the lungs to various tissues and the transfer of carbon dioxide from tissues back to the lungs. Additionally, hemoglobin plays a significant role in maintaining the pH … Read more

Blood Cross Matching – Principle, Procedure, Result.

Cross Match

Cross Match is a laboratory test, performed prior to a blood transfusion to determine whether donor blood is compatible (or incompatible) with recipient blood.

Polymerase Chain Reaction (PCR) – Definition, Steps, Principle, Application

Polymerase chain reaction Steps

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).

Blood Grouping Test – Principle, Procedure, Result

Blood Grouping Principle and Procedure

The concept of Blood grouping was first discovered by Karl Landsteiner in 1901, who was an Austrian-American immunologist and pathologist. He received Nobel Prize in Physiology or Medicine in 1930 for this discovery.

Gel Staining Procedure for PAGE

Gel Staining Procedure for PAGE

Staining protein gels using Coomassie Brilliant Blue R-250 is common to see proteins that are resolved using SDS-PAGE. It is extremely sensitive and suitable for long-term storage of gels.

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