Air Displacement Pipetting – Principle, Procedure, Applications

Air Displacement Pipetting - Principle, Procedure, Applications

An air displacement pipette (also known as a “volumetric pipette” or “mechanical pipette”) is a type of liquid handling tool commonly used in laboratory settings. It uses air displacement to draw a specific volume of liquid into the pipette’s barrel. The volume of liquid is determined by the calibrated volume markings on the barrel of … Read more

How to Use a Micropipette?

How to Use a Micropipette?

Standard laboratory equipment for measuring and transferring minute amounts of liquid includes micropipettors. You’ll put them to use all through the semester and into subsequent upper-level courses. If you want reliable results from your tests, you must become an expert user of these tools. Objective Parts of a micropipette Different sizes of micropipettes The laboratory’s … Read more

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa on Macconkey Agar

Pseudomonas Aeruginosa Principle The MacConkey Agar is a specialized culture medium widely used in microbiology for the isolation, identification, and differentiation of gram-negative bacteria, particularly those belonging to the Enterobacteriaceae family. The principle of MacConkey Agar lies in its combination of selective and differential properties, making it an effective tool in microbiological analyses. The medium’s … Read more

Microinjection – Definition, Types, Principle, Steps Applications

Microinjection - Definition, Types, Principle, Steps Applications

What is Microinjection? Definition of Microinjection Microinjection is a precise technique used to introduce DNA or other genetic materials directly into a cell using a micropipette or fine glass needle. It is widely employed in genetic engineering, genome editing, and various biomedical research areas. Principle of Microinjection Microinjection operates on the fundamental principle of directly … Read more

Filtration – Definition, Mechanisms, Types, Examples, Application

Filtration - Definition, Mechanisms, Types, Examples, Application

What is Filtration? Filtration is utilised to separate a dispersion based on particle size. The filtered mixture is put to an appropriate porous material filter. Particles smaller than the filter’s pores can pass through and enter the filtrate, while larger particles are retained on the filter’s surface. Filtration is a process that is used to … Read more

Method for Balancing in Laboratory – Weight or Mass Measurement

Method for Balancing in Laboratory - Weight or Mass Measurement

The most frequent tasks in the microbiology area is the determination of the weight or mass of desired substances, chemicals. Another important aspect to be considered is the preservation of substances. Thus, the powders, as well as other granular or paste-like substances should Chamber not be placed directly on the platform for weighing of the balance. It is recommended that glazed paper or a small weigh boats is the best choice to weigh. It is recommended to use glazed papers if the material is 15 grams or less needs to be weighed. A the weigh boat pan or small beaker must be utilized if you are weighing a larger amount. Because of the the light weight of glazed papers its weight is subject to a minimal. If the weight is less of 1 gram an electronic balance is recommended. The larger amount (above 1 grams) is not to be considered using electronic balances.

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Preparation Of Temporary Cotton Plugs and Permanent Cotton Plugs

Microorganisms are everywhere in their the distribution. In any given environment, many microorganisms are present at any given time. It is difficult to identify a specific kind of microbe until we understand their ecological needs. For instance, anaerobic microbes do not require oxygen those that are aerobic require oxygen micro-aerophiles to have oxygen however, in a small amount. So, we require cotton plugs to provide an conditions that are aerobic and keep the growing culture uninvolved with unwanted microbes. Cotton plugs are made of cotton along with air pours. Air can flow through air pours, but not the bacteria that cause microbial contamination. They are fixed to the surface of cotton fibres , and prevent them from being able from getting into flasks, tubes, etc. Air aids in for the development of microorganisms within glass equipment.

Isolation of Microorganisms from Soil

Isolation of Microorganism from Soil

Soil contain different types of microorganisms which are listed below; Principle of Isolation of Microorganisms from Soil During this process, the soil sample is collected from the study site. After that, the soil sample is dissolved in distilled water and then serial dilution is performed. Then the spread-plate/streak plate method is performed on the sample … Read more

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