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Cellulose Acetate Electrophoresis – Definition, Principle, Operating Procedure, Uses

Cellulose Acetate Electrophoresis - Definition, Principle, Operating Procedure, Uses

Principle of Cellulose Acetate Electrophoresis Materials Required for Cellulose Acetate Electrophoresis Steps / Procedure of Cellulose Acetate Electrophoresis Cellulose Acetate as Electrophoretic Support Matrix Application of Cellulose Acetate Electrophoresis Advantages of Cellulose Acetate Electrophoresis Limitation of Cellulose Acetate Electrophoresis Comparisons with Gel or Capillary Electrophoresis FAQ on Cellulose Acetate Electrophoresis

Compound Microscope – Principle, Parts, Diagram Definition, Application

Compound Microscope - Principle, Parts, Diagram Definition, Application

What is a Compound Microscope? 3D Model of Compound Microscope Working Principle of Compound Microscope Magnification Power of Compound Microscope The magnification power of compound microscope refers to ratio of size of final image to size of object seen by naked eye. It is obtained by multiplying magnification of objective lens and magnification of eyepiece … Read more

Pulsed-field Gel Electrophoresis (PFGE) – Definition, Types, Steps, Applications

Pulsed-field Gel Electrophoresis (PFGE) - Definition, Types, Steps, Applications

What is Pulsed-field Gel Electrophoresis (PFGE)? Pulsed Field Gel Electrophoresis (PFGE) is a method used for separation of very large DNA fragments by applying an electric field that periodically changes direction / switches among different electrodes, so that DNA pieces of differing sizes are reoriented and separated. (The technique allows resolution of DNA up to … Read more

Applications of Fluorescence Spectroscopy

Applications of Fluorescence Spectroscopy

Fluorescence Spectroscopy is an analytical method that studies the fluorescence emitted by a sample when it is excited by light (often UV or visible). Principle of Fluorescence Spectroscopy Applications of Fluorescence Spectroscopy in inorganic chemistry Special fluorimetric applications

Water bath – Principle, Types, Parts, Operating Procedure, Uses

Laboratory Water bath - Principle, Types, Parts, Operating Procedure, Uses

What is a Water Bath? A Laboratory Water Bath can define as equipment used for maintaining constant temperature condition for samples during experiment or incubation process. It is basically consist of container filled with water, which heated to desired temperature and samples are placed inside for uniform heating purpose. The principle of operation based on … Read more

Incubator – Definition, Principle, Parts, Types, Procedure, Use

Incubator - Definition, Principle, Components, Types, Operating Procedure, Use.

What is Laboratory Incubator? A Laboratory Incubator is described as a device that is used to grow and maintain microbial / cell cultures under controlled temperature, humidity, and gas conditions. The environment inside is usually kept stable, and temperature is controlled (often by thermostats/sensors), though fluctuations are caused by door openings. For mammalian cells, a … Read more

Autoclave – Definition, Principle, Parts, and Uses Explained

Autoclave - Definition, Principle, Parts, Operating Procedure, Uses

What is an autoclave? An autoclave is a device that is used for sterilization / disinfection by means of steam under pressure, and it is found in hospitals, labs, industry. Items are placed in a chamber where they are exposed to high-pressure saturated steam (commonly 121 °C for 15–20 minutes) so that microbes and spores … Read more

Polymerase Chain Reaction (PCR) – Definition, Steps, Principle, Application

Polymerase chain reaction Steps

Polymerase chain reaction or PCR is a laboratory technique that is used to make multiple copies (millions or billions!) of a specific region of DNA in vitro (in a test tube rather than an organism).

Reverse Transcription PCR (RT-PCR) – Principle, Steps, Applications

COVID-19 Diagnostic Test through RT-PCR

What is Reverse Transcription PCR (RT-PCR)? Reverse Transcription Polymerase Chain Reaction (RT-PCR) is defined as a lab method in which RNA is converted into complementary DNA (cDNA) by an enzyme called reverse transcriptase, then that cDNA is amplified via PCR. The technique is used for detection, quantification of specific RNA molecules (eg mRNA, viral RNA) … Read more

Repetitive sequence-based PCR (rep-PCR) – Principle, Steps, Uses

Repetitive sequence-based PCR (rep-PCR) - Principle, Steps, Uses

What is Repetitive sequence-based PCR (rep-PCR)? Working Principle of rep-PCR The rep-PCR working principle is based on the occurrence of conserved repetitive DNA elements (REP, ERIC, BOX, GTG5 etc.), which are dispersed at multiple sites within prokaryotic genomes, and these elements serve as priming sites for amplification. By the use of specifically designed primers, annealing … Read more

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