Collagen Hybridizing Peptide Staining – Principle, Procedure, Uses
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
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Kinyoun stain (Acid Fast Cold) Method – Principle, Procedure, Result
Kinyoun method also known as Kinyoun stain (cold method), was first developed by Joseph J. Kinyoun.
Ziehl-Neelsen Staining (ZN Staining) – Principle and Procedure, Result
Ziehl Neelsen stinging method is used to differentiate between the acid-fast and non-acid fast bacteria.
Lactophenol Cotton Blue (LPCB) Staining – Principle, Procedure, Result.
Lactophenol Cotton Blue (LPCB) staining is the simple wet-mount method used for microscopic examination of fungi, and it is considered one of the common procedure in mycology. It is the process where fixation, staining, and preservation of fungal structures is achieved within a single reagent. The solution is prepared by phenol, lactic acid, glycerol and … Read more
Sudan Black B Staining – Principle, Procedure, Uses
Sudan Black is a slightly basic dye and will combine with acidic groups in compound lipids, thus staining phospholipids also. An alternative stain to the Sudan Black B stain.
Isolation of Bacillus thuringiensis (Bt) from Soil sample
Bacillus thuringiensis (Bt) is a Gram-positive, facultatively anaerobic bacterium that belongs to the B. cereus group. It is the organism that produces the proteinaceous parasporal crystals during sporulation, and these crystals are called delta-endotoxins. These are known as Cry and Cyt toxins, and it is the major insecticidal component. It is widely distributed in soil, … Read more
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
Flagella Staining – Principle, Procedure, Result
Flagella can not be seen under a bright-field microscope by using ordinary stains. A simple and useful method has been used for visualization of flagella is known as wet mount technique.
Leifson Technique for Bacterial Flagella Staining
In 1930, Leifson introduced a simple flagella stain, using this stain he observed that mutations leading to nonflagellated from flagellated bacteria were common, as well as mutation leading to loss of motility without loss of the flagellum itself.
Thick and Thin Blood Smear Preparation
A blood smear is a simple diagnostic preparation where a drop of peripheral blood is spread on a clean slide, and it is dried for staining. It is the process used for observing blood cells and also for detecting parasites after staining methods like Giemsa. It is an important technique because it helps in demonstrating … Read more