Culture Media Examples Components/Comments Primary Purpose Bile esculin agar (BEA) Nutrient agar base with ferric citrate. Hydrolysis of esculin by group D streptococci imparts a brown color to medium; sodium deoxycholate inhibits many bacteria. Differential isolation and presumptive identification of group D streptococci and enterococci Bile esculin azide agar with vancomycin Contains azide to inhibit … Read more
What is Columbia CNA Agar? Columbia CNA Agar with 5% Sheep Blood Composition of Columbia CNA Agar per Liter of Medium The composition of Columbia CNA Agar can be summarized as follows, considering the information provided: Ingredients: The base of Columbia CNA Agar is prepared using a nutritionally rich medium that includes different peptones to … Read more
Culture media refers to the nutrient-rich substances used in laboratories to cultivate microorganisms such as bacteria and fungi. These substances, which may be liquid or solid, give the microbes with the required nutrients, minerals, and other elements for growth and reproduction. In the late 19th century, when scientists first began to study microorganisms in depth, … Read more
What is Bile Esculin Agar (BEA)? Bile Esculin Agar (BEA) Composition Bile Esculin Agar (BEA) contains the following ingredients per liter of the medium: The final pH of the medium, when measured at 25°C, is approximately 6.6±0.2. These specific components and their concentrations are carefully selected to create a medium that is selective and differential … Read more
What is Columbia Blood Agar Base? Principle of Columbia Blood Agar Base The principle of Columbia Blood Agar Base lies in its composition and functionality in supporting the growth and differentiation of various bacterial species. Here are the key principles of Columbia Blood Agar Base: Composition of Columbia Blood Agar Base Ingredients Gms/Litre Peptone special … Read more
What is Simmons Citrate Agar? Composition of Simmons Citrate Agar Ingredients Gms/liter Magnesium sulfate 0.200 Ammonium dihydrogen phosphate 1.000 Dipotassium phosphate 1.000 Sodium citrate 2.000 Sodium chloride 5.000 Bromothymol blue 0.080 Agar 15.000 Final pH ( at 25°C) 6.8±0.2 Principle of Simmons Citrate Agar The principle of Simmons Citrate Agar revolves around the ability of … Read more
Transport media are basically buffer solutions that contain carbohydrate, peptones, along with other nutritional elements (excluding growing factors) that are designed to protect the health of bacteria during transport, without allowing for their multiplicity. The main goal of utilization of the medium is to keep the sample as close to its original condition as is possible.
Burkholderia cepacia can be viewed as an opportunity-based bacterium that is associated with nosocomial illnesses caused by contamination of medical equipment and disinfectants. However, the highest risk group is CF patients. People with cystic fibrosis are at an increased risk of infection. those with the infection, if not treated are prone to rapid declines of lung functions, frequent bacteremia and even death due to lung dysfunction.