The GasPak Anaerobic System is a laboratory method used to grow bacteria that thrive in oxygen-free environments, known as anaerobes. Using throwaway sachets filled with compounds like sodium borohydride and sodium bicarbonate helps to create an anaerobic (oxygen-free) environment more quickly. These molecules combine to generate hydrogen and carbon dioxide when water is introduced. The … Read more
What is BOD Incubator? BOD incubator Definition The BOD (Biological Oxygen Demand) Incubator is a specialized laboratory instrument designed to provide a controlled temperature environment, typically around 20 degrees centigrade, for microbial and biochemical studies, particularly for assessing the oxygen demand of microorganisms in water samples. BOD incubator Principle Operating on a well engineered mechanism, … Read more
Differential centrifugation (or the differential velocity method) is one of the most prevalent techniques applied in biochemistry and cell biology regarding the separation of organelles and other subcellular particles according to their sedimentation rates. It is available not only for separate microscopic analyses, but also as a useful technique for preliminary remediation of suspended nonliving … Read more
What is an Autoclave Bag? Autoclave bags are made of specific materials that can withstand high temperatures and pressures. Autoclave bags, usually filled with polypropylene, are used in medical/laboratory environments and procedures to sterilize contaminated waste for proper and safe disposal. Autoclave bags are designed to contain biohazard waste and make them biohazard safe. These … Read more
What is a Density Gradient Centrifugation? Scientists apply density gradient centrifugation in laboratories to sort particles including cells, organelles, and biomolecules because of their density differences. During density gradient centrifugation a sample sits on top of a density gradient medium formed by substances such as sucrose or cesium chloride. During high-speed centrifuge spinning particles from … Read more
What is Laminar flow hood/cabinet? A laminar flow hood, or laminar flow cabinet, is a type of work environment used in pharmaceutical, medical, and research laboratories that creates a well regulated, sterilised environment when working with delicate material or processes. The main job of a laminar flow hood is to maintain an area free of contaminates by … Read more
Ion exchange chromatography (IEC) is a chromatographic method that exploits the charge properties of the molecules. It employs an insoluble matrix, which is covalently bonded with a charged group, as a stationary phase. These groups will then also have the ability to attract and bind ions that have the opposite charge and that are in the mobile phase, … Read more
What is Dark Field Microscopy? Darkfield microscopy is a form of light microscopy that facilitates detection by contrast. For example, brightfield microscopy uses a light source that shines light directly through the sample in order to illuminate the sample from below; darkfield microscopy uses a condenser that shines light at an oblique angle so that … Read more
What is Fluorescence Microscopy? A fluorescence microscope is an optical microscope that employs fluorescence for examining specific properties of select organic and inorganic substances. A fluorescence microscope functions by observing labeled samples; the labels here are the specific fluorescent molecules (or clusters of molecules)—termed fluorophores—that emit light when stimulated at specific frequencies. Components of a … Read more
The oil immersion method enhances the resolving power of a microscope, enabling light microscopy to distinguish finer details. Immersion oil—clear and colorless, having a refractive index of about 1.515—is placed in between the lens and the specimen. The refractive index is higher; therefore, less light is refracted. If less light is refracted, then less is … Read more
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