Leucine aminopeptidase (LAP) is an enzyme used to distinguish catalase-negative, gram-positive cocci of Aerococcus and Leuconostoc (LAP test negative) from other organisms in the group, such as Streptococcus, Enterococcus, Lactococcus, and Pediococcus (almost always positive).
A lipase test is a laboratory diagnostic test which is done to measure the level of lipase enzyme in the blood. Lipase is a digestive enzyme which is mainly produced by pancreas and it helps in digestion of fats. It is the process in which dietary triglycerides are broken down into fatty acids and glycerol … Read more
Simmons Citrate Agar is a chemically defined medium in which sodium citrate act as the only carbon source and ammonium dihydrogen phosphate act as the only nitrogen source. It is the medium developed as a modification of Koser’s citrate medium and it is used mainly to differentiate members of Enterobacteriaceae. It is the process in … Read more
CAMP Test was first discovered by four researchers Christie, Atkins, Munch, and Peterson in 1944, that’s why this test is also known as Christie–Atkins–Munch-Peterson test.
In this method, the Group B Streptococcus (CAMP test positive) is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it.
It is a type of biochemical test which is used to distinguish reducing sugars from non-reducing sugars. This biochemical test is also known as the silver mirror test based on the end product of this test. This test was also used to differentiate between aldehydes and ketones through routine qualitative organic analysis.
The oxidase test is a biochemical reaction that assays for the presence of cytochrome oxidase, an enzyme sometimes called indophenol oxidase. In the presence of an organism that contains the cytochrome oxidase enzyme, the reduced colorless reagent becomes an oxidized colored product .
Cell membrane staining is the process in which the plasma membrane of a cell is visualized with the help of dyes or fluorescent probes. It is the thin outer covering of the cell, and it is normally transparent, so it cannot be seen clearly under a simple light microscope. It is the process where special … Read more
Giemsa stain is a polychromatic nucleic acid stain that is used mainly for demonstrating different blood cells and parasites in stained blood films. It is named after the German chemist Gustav Giemsa. It belongs to a group of stains known as Romanowsky stains. These are neutral stains made from a mixture of oxidized methylene blue, … Read more
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
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