Southern Blot – Definition, Principle, Steps, Importance
Southern Blotting is a molecular biology technique used for the identification of a specific DNA sequence in a collected DNA sample.
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Gerhardt’s test – Principle, Procedure, Result
Gerhardt’s test is a type of laboratory test which is performed for the qualitative detection of ketone bodies in urine.
Nuclear Staining by Giemsa stain – Procedure, Principle, Result
Bacterial cells contain nuclear material, made of single-stranded circular DNA, in contrast to eukaryotes. This genetic material is present within the membrane-bounded structure known as the nucleus. In case of prokaryotes the nuclear material is present within the nucleoid which lacks the nuclear membrane and does not follow the mitosis or miosis cell division. Some … Read more
Cell Membrane Staining Procedure, Principle, Result
The cell membrane is a biological membrane which also termed a plasma membrane (PM) or cytoplasmic membrane. It divides the interior of the cell from the outside environment, thus protects the cell from environmental stress. The cell membrane is a lipid bilayer, contains cholesterols between phospholipids which maintain their fluidity at various temperatures. The membrane … Read more
Collagen Hybridizing Peptide Staining
Collagen Hybridizing Peptide stain is a vital stain used for the detection of collagen degraded tissues, mainly used in Developmental Biology, Histology, and Histopathology.
Iron-Hematoxylin Staining – Principle, Procedure, Result
Hematoxylin is extracted from a leguminous plant, known as Haematoxylon campechianum, thus it is a natural dye. It forms hematein by oxidation (Ripening).
Acridine Orange Staining
Acridine Orange contains metachromatic properties, that’s why it is employed in fluorescence microscopy and flow cytometry study of cellular physiology and cell cycle state, including the fluorescent microscopic examination of microorganisms.
Capsule Staining – Procedure, Principle, Result
Capsule staining is a differential staining method, where two different stains are used such as the primary stain and the counterstain.
Parasporal Crystal Stain – Principle, Procedure, Result
Production of parasporal crystals is a unique ability (among Bacillus species) of Bacillus thuringiensis. This stain is a means of rapid identification of the species.
Latex agglutination test – Definition, Procedure, Principle, Advantages, Limitation, Uses
What is the latex agglutination test? Types of Latex Agglutination The latex agglutination test can be categorized into two main types based on the processes of detection: Latex Agglutination Test (LAT) for Antibody Detection and Latex Agglutination Test (LAT) for Antigen Detection. These two types of latex agglutination tests provide valuable diagnostic information in different … Read more